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dc.contributor.authorAlsos, Inger Greve
dc.contributor.authorLammers, Youri
dc.contributor.authorYoccoz, Nigel Gilles
dc.contributor.authorJørgensen, Tina
dc.contributor.authorSjögren, Per
dc.contributor.authorGielly, Ludovic
dc.contributor.authorEdwards, Mary E.
dc.date.accessioned2018-08-17T08:16:38Z
dc.date.available2018-08-17T08:16:38Z
dc.date.issued2018-04-17
dc.description.abstractMetabarcoding of lake sediments have been shown to reveal current and past biodiversity, but little is known about the degree to which taxa growing in the vegetation are represented in environmental DNA (eDNA) records. We analysed composition of lake and catchment vegetation and vascular plant eDNA at 11 lakes in northern Norway. Out of 489 records of taxa growing within 2 m from the lake shore, 17–49% (mean 31%) of the identifiable taxa recorded were detected with eDNA. Of the 217 eDNA records of 47 plant taxa in the 11 lakes, 73% and 12% matched taxa recorded in vegetation surveys within 2 m and up to about 50 m away from the lakeshore, respectively, whereas 16% were not recorded in the vegetation surveys of the same lake. The latter include taxa likely overlooked in the vegetation surveys or growing outside the survey area. The percentages detected were 61, 47, 25, and 15 for dominant, common, scattered, and rare taxa, respectively. Similar numbers for aquatic plants were 88, 88, 33 and 62%, respectively. Detection rate and taxonomic resolution varied among plant families and functional groups with good detection of e.g. Ericaceae, Roseaceae, deciduous trees, ferns, club mosses and aquatics. The representation of terrestrial taxa in eDNA depends on both their distance from the sampling site and their abundance and is sufficient for recording vegetation types. For aquatic vegetation, eDNA may be comparable with, or even superior to, in-lake vegetation surveys and may therefore be used as an tool for biomonitoring. For reconstruction of terrestrial vegetation, technical improvements and more intensive sampling is needed to detect a higher proportion of rare taxa although DNA of some taxa may never reach the lake sediments due to taphonomical constrains. Nevertheless, eDNA performs similar to conventional methods of pollen and macrofossil analyses and may therefore be an important tool for reconstruction of past vegetation.en_US
dc.descriptionThis is the final version of the following article: Alsos, I.G., Lammers, Y., Yoccoz, N.G., Jørgensen, T., Sjögren, P., Gielly, L. & Edwards, M.E. (2018). Plant DNA metabarcoding of lake sediments: How does it represent the contemporary vegetation. PLoS ONE, 13(4). https://doi.org/10.1371/journal.pone.0195403, which can be retrieved from <a href=https://doi.org/10.1371/journal.pone.0195403> https://doi.org/10.1371/journal.pone.0195403</a>. Licensed <a href=http://creativecommons.org/licenses/by-nc-nd/4.0/> CC BY-NC-ND 4.0.</a>en_US
dc.identifier.citationAlsos, I.G., Lammers, Y., Yoccoz, N.G., Jørgensen, T., Sjögren, P., Gielly, L. & Edwards, M.E. (2018). Plant DNA metabarcoding of lake sediments: How does it represent the contemporary vegetation. PLoS ONE, 13(4). https://doi.org/10.1371/journal.pone.0195403en_US
dc.identifier.cristinIDFRIDAID 1583395
dc.identifier.issn1932-6203
dc.identifier.otherPlant DNA metabarcoding of lake sediments: How does it represent the contemporary vegetation
dc.identifier.urihttps://hdl.handle.net/10037/13448
dc.language.isoengen_US
dc.publisherPublic Library of Scienceen_US
dc.relation.ispartofLammers, Y. (2020). Sedimentary ancient DNA: Exploring methods of ancient DNA analysis for different taxonomic groups. (Doctoral thesis). <a href=https://hdl.handle.net/10037/17437>https://hdl.handle.net/10037/17437. </a>
dc.relation.journalPLoS ONE
dc.relation.projectIDinfo:eu-repo/grantAgreement/RCN/FRIMEDBIO/213692/Norway/Ancient DNA of NW Europe reveals responses to climate change//en_US
dc.relation.projectIDinfo:eu-repo/grantAgreement/RCN/FRIMEDBIO/250963/Norway/Ecosystem change and species persistence over time: a genome-based approach/ECOGEN/en_US
dc.relation.projectIDinfo:eu-repo/grantAgreement/RCN/KLIMAFORSK/230617/Norway/Ancient DNA of NW Europe reveals responses to climate change//en_US
dc.rights.accessRightsopenAccessen_US
dc.subjectVDP::Matematikk og Naturvitenskap: 400::Zoologiske og botaniske fag: 480en_US
dc.subjectVDP::Mathematics and natural science: 400::Zoology and botany: 480en_US
dc.subjectLakesen_US
dc.subjectSedimenten_US
dc.subjectPolymerase chain reactionen_US
dc.subjectSequence databasesen_US
dc.subjectDNA filter assayen_US
dc.subjectTaxonomyen_US
dc.subjectAncient DNAen_US
dc.subjectPollenen_US
dc.titlePlant DNA metabarcoding of lake sediments: How does it represent the contemporary vegetationen_US
dc.typeJournal articleen_US
dc.typeTidsskriftartikkelen_US
dc.typePeer revieweden_US


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