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dc.contributor.authorØie, Cristina Ionica
dc.contributor.authorMönkemöller, Viola
dc.contributor.authorHübner, Wolfgang
dc.contributor.authorSchüttpelz, Mark
dc.contributor.authorMao, Hong
dc.contributor.authorAhluwalia, Balpreet Singh
dc.contributor.authorHuser, Thomas Rolf
dc.contributor.authorMcCourt, Peter Anthony
dc.date.accessioned2018-09-07T12:47:15Z
dc.date.available2018-09-07T12:47:15Z
dc.date.issued2018-01-10
dc.description.abstractSuper-resolution fluorescence microscopy, also known as nanoscopy, has provided us with a glimpse of future impacts on cell biology. Far-field optical nanoscopy allows, for the first time, the study of sub-cellular nanoscale biological structures in living cells, which in the past was limited to electron microscopy (EM) (in fixed/dehydrated) cells or tissues. Nanoscopy has particular utility in the study of “fenestrations” – phospholipid transmembrane nanopores of 50–150 nm in diameter through liver sinusoidal endothelial cells (LSECs) that facilitate the passage of plasma, but (usually) not blood cells, to and from the surrounding hepatocytes. Previously, these fenestrations were only discernible with EM, but now they can be visualized in fixed and living cells using structured illumination microscopy (SIM) and in fixed cells using single molecule localization microscopy (SMLM) techniques such as direct stochastic optical reconstruction microscopy. Importantly, both methods use wet samples, avoiding dehydration artifacts. The use of nanoscopy can be extended to the <i>in vitro</i> study of fenestration dynamics, to address questions such as the following: are they actually dynamic structures, and how do they respond to endogenous and exogenous agents? A logical further extension of these methodologies to liver research (including the liver endothelium) will be their application to liver tissue sections from animal models with different pathological manifestations and ultimately to patient biopsies. This review will cover the current state of the art of the use of nanoscopy in the study of liver endothelium and the liver in general. Potential future applications in cell biology and the clinical implications will be discussed.en_US
dc.description.sponsorshipThe Tromsø Research Foundation UiT The Arctic University of Norway The German Academic Exchange Service The DFG The Ministry of Innovation, Science, Research and Technology of the State of North Rhine-Westphalia (MIWFT)en_US
dc.descriptionSource at <a href=https://doi.org/10.1515/nanoph-2017-0055> https://doi.org/10.1515/nanoph-2017-0055</a>.en_US
dc.identifier.citationØie, C.I., Mönkemöller, V., Hübner, W., Schüttpelz, M., Mao, H., Ahluwalia, B.S., ... McCourt, P. (2018). New ways of looking at very small holes – using optical nanoscopy to visualize liver sinusoidal endothelial cell fenestrations. Nanophotonics, 7(3). https://doi.org/10.1515/nanoph-2017-0055en_US
dc.identifier.cristinIDFRIDAID 1549933
dc.identifier.doihttps://doi.org/10.1515/nanoph-2017-0055
dc.identifier.issn2192-8606
dc.identifier.issn2192-8614
dc.identifier.urihttps://hdl.handle.net/10037/13717
dc.language.isoengen_US
dc.publisherDe Gruyter Openen_US
dc.relation.journalNanophotonics
dc.relation.projectIDinfo:eu-repo/grantAgreement/EC/FP7-IDEAS-ERC/336716/EU/High-speed chip-based nanoscopy to discover real-time sub-cellular dynamics/NANOSCOPY/en_US
dc.relation.projectIDinfo:eu-repo/grantAgreement/EC/H2020-EU.1.3.1. - Fostering new skills by means of excellent initial training of researchers/766181/EU/Super-resolution optical microscopy of nanosized pore dynamics in endothelial cells/DeLIVER/en_US
dc.relation.projectIDinfo:eu-repo/grantAgreement/RCN/IS-DAAD/244764/Norway/Integrated Optical Nanoscopy//en_US
dc.relation.urihttps://www.degruyter.com/view/j/nanoph.ahead-of-print/nanoph-2017-0055/nanoph-2017-0055.xml
dc.rights.accessRightsopenAccessen_US
dc.subjectVDP::Teknologi: 500::Nanoteknologi: 630en_US
dc.subjectVDP::Technology: 500::Nanotechnology: 630en_US
dc.subjectliveren_US
dc.subjectendotheliumen_US
dc.subjectoptical nanoscopyen_US
dc.subjectfenestrationen_US
dc.titleNew ways of looking at very small holes – using optical nanoscopy to visualize liver sinusoidal endothelial cell fenestrationsen_US
dc.typeJournal articleen_US
dc.typeTidsskriftartikkelen_US
dc.typePeer revieweden_US


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