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dc.contributor.authorAjayi, Clement
dc.contributor.authorÅberg, Espen
dc.contributor.authorAskarian, Fatemeh
dc.contributor.authorSollid, Johanna U Ericson
dc.contributor.authorJohannessen, Mona
dc.contributor.authorHanssen, Anne Merethe
dc.date.accessioned2018-10-24T13:18:04Z
dc.date.available2018-10-24T13:18:04Z
dc.date.issued2018-04-16
dc.description.abstract<p><i>Background</i>: <i>Staphylococcus aureus</i> cell wall anchored Serine Aspartate repeat containing protein D (<i>sdrD</i>) is a member of the microbial surface component recognising adhesive matrix molecules (MSCRAMMs). It is involved in the bacterial adhesion and virulence. However the extent of genetic variation in <i>S. aureus sdrD</i> gene within isolates from healthy carriers are not known. The aim of this study was to evaluate allelic variation of the <i>sdrD</i>gene among <i>S. aureus</i> from healthy nasal carriers.</p> <p><i>Results</i>: The <i>sdrD</i> A region from 48 <i>S. aureus</i> isolates from healthy carriers were analysed and classified into seven variants. Variations in the <i>sdrD</i> A region were concentrated in the N2 and N3 subdomains. Sequence analysis of the entire <i>sdrD</i> gene of representative isolates revealed variations in the SD repeat and the EF motifs of the B repeat. In silico structural modelling indicates that there are no differences in the <i>sdrD</i> structure of the 7 variants. Variable amino acid residues mapped onto the 3D structure revealed that the variations are surface located, exist within the groove between the N2-N3 subdomains and distributed mainly on the N3 subdomain. Comparison of adhesion to keratinocytes in an in vitro cell adhesion assay, using NCTC 8325–4∆<i>sdrD</i> strains expressing the various <i>sdrD</i> gene variants, indicated a significant difference between only two complements while others showed no major difference in their adhesion.</p> <p><i>Conclusions</i>: This study provides evidence of sequence variations across the different domains of <i>sdrD</i> from <i>S. aureus</i> isolated from healthy nasal carriers. Proper understanding of these variations is necessary in the study of <i>S. aureus</i> pathogenesis.en_US
dc.description.sponsorshipThe Northern Norway Regional Health Authority UiT The Arctic University of Norway.en_US
dc.descriptionSource at <a href=https://doi.org/10.1186/s12866-018-1179-7> https://doi.org/10.1186/s12866-018-1179-7</a>. Licensed <a href=http://creativecommons.org/licenses/by-nc-nd/4.0/> CC BY-NC-ND 4.0.</a>en_US
dc.identifier.citationAjayi, C., Åberg, E., Askarian, F., Sollid, J.U.E., Johannessen, M. & Hanssen, A.-M. (2018). Genetic variability in the sdrD gene in Staphylococcus aureus from healthy nasal carriers. BMC Microbiology, 18(34). https://doi.org/10.1186/s12866-018-1179-7en_US
dc.identifier.cristinIDFRIDAID 1585057
dc.identifier.doi10.1186/s12866-018-1179-7
dc.identifier.issn1471-2180
dc.identifier.urihttps://hdl.handle.net/10037/14031
dc.language.isoengen_US
dc.publisherBMCen_US
dc.relation.ispartofAjayi, C. (2018). Determinants of Staphylococcus aureus Colonization and Infection. “Exploring the Role of Cell Wall Anchored Proteins in Adhesion and Immune Evasion”. Doctoral thesis. <a href=http://hdl.handle.net/10037/14070>http://hdl.handle.net/10037/14070</a>
dc.relation.journalBMC Microbiology
dc.relation.projectIDinfo:eu-repo/grantAgreement/RCN/FORSKSKOLE /249062/Norway/Norwegian Research School in Infection Biology and Antimicrobials (IBA)//en_US
dc.rights.accessRightsopenAccessen_US
dc.subjectVDP::Mathematics and natural science: 400::Basic biosciences: 470::General immunology: 478en_US
dc.subjectVDP::Matematikk og Naturvitenskap: 400::Basale biofag: 470::Generell immunologi: 478en_US
dc.subjectS. aureusen_US
dc.subjectSdren_US
dc.subjectHealthy carrieren_US
dc.subjectVirulence factorsen_US
dc.subjectAllelic variantsen_US
dc.titleGenetic variability in the sdrD gene in Staphylococcus aureus from healthy nasal carriersen_US
dc.typeJournal articleen_US
dc.typeTidsskriftartikkelen_US
dc.typePeer revieweden_US


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