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dc.contributor.authorLomet, Didier
dc.contributor.authorPiegu, Benoit
dc.contributor.authorWood, Shona Hiedi
dc.contributor.authorDardente, Hugues
dc.date.accessioned2018-11-23T13:46:44Z
dc.date.available2018-11-23T13:46:44Z
dc.date.issued2018-05-10
dc.description.abstractThis study investigated Vegfa expression in the pars tuberalis (PT) of the pituitary and medio-basal hypothalamus (MBH) of sheep, across seasons and reproductive states. It has recently been proposed that season impacts alternative splicing of Vegfa mRNA in the PT, which shifts the balance between angiogenic VEGFAxxx and anti-angiogenic VEGFAxxxb isoforms (with xxx the number of amino acids of the mature VEGFA proteins) to modulate seasonal breeding. Here, we used various RT-PCR methodologies and analysis of RNAseq datasets to investigate seasonal variation in expression and splicing of the ovine Vegfa gene. Collectively, we identify 5 different transcripts for Vegfa within the ewe PT/MBH, which correspond to splicing events previously described in mouse and human. All identified transcripts encode angiogenic VEGFAxxx isoforms, with no evidence for alternative splicing within exon 8. These findings led us to investigate in detail how “Vegfaxxxb-like” PCR products could be generated by RT-PCR and misidentified as endogenous transcripts, in sheep and human HEK293 cells. In conclusion, our findings do not support the existence of anti-angiogenic VEGFAxxxb isoforms in the ovine PT/MBH and shed new light on the interpretation of prior studies, which claimed to identify Vegfaxxxb isoforms by RT-PCR.en_US
dc.descriptionSource at <a href=https://doi.org/10.1371/journal.pone.0197123> https://doi.org/10.1371/journal.pone.0197123</a>.en_US
dc.identifier.citationLomet, D., Piégu, B., Wood, S.H. & Dardente, H. (2018). Anti-angiogenic VEGFAxxxb transcripts are not expressed in the medio-basal hypothalamus of the seasonal sheep. <i>PLoS ONE</i>, 13(5). https://doi.org/10.1371/journal.pone.0197123en_US
dc.identifier.cristinIDFRIDAID 1623255
dc.identifier.doi10.1371/journal.pone.0197123
dc.identifier.issn1932-6203
dc.identifier.urihttps://hdl.handle.net/10037/14230
dc.language.isoengen_US
dc.publisherPublic Library of Scienceen_US
dc.relation.journalPLoS ONE
dc.relation.projectIDinfo:eu-repo/grantAgreement/EC/FP7-PEOPLE/320553/EU/Deciphering the role of thyroid hormones in seasonal reproduction/THYROREPRO/en_US
dc.rights.accessRightsopenAccessen_US
dc.subjectVDP::Mathematics and natural science: 400::Basic biosciences: 470en_US
dc.subjectVDP::Matematikk og Naturvitenskap: 400::Basale biofag: 470en_US
dc.titleAnti-angiogenic VEGFAxxxb transcripts are not expressed in the medio-basal hypothalamus of the seasonal sheepen_US
dc.typeJournal articleen_US
dc.typeTidsskriftartikkelen_US
dc.typePeer revieweden_US


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