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dc.contributor.authorGrip, Jostein
dc.contributor.authorEngstad, Rolf Einar
dc.contributor.authorSkjæveland, Ingrid
dc.contributor.authorSkalko-Basnet, Natasa
dc.contributor.authorIsaksson, Johan
dc.contributor.authorBasnet, Purusotam
dc.contributor.authorHolsæter, Ann Mari
dc.date.accessioned2019-01-22T09:39:17Z
dc.date.available2019-01-22T09:39:17Z
dc.date.issued2018-06-01
dc.description.abstractThe increased prevalence of chronic wounds requires novel treatment options. The aim of this study was to develop a beta-glucan (βG)-loaded nanofiber wound dressing. Nanofibers were prepared using the needle-free Nanospider™ technology, an electrospinning method which enables the production of nanofibers at an industrial scale. The βG was selected as active ingredient based on its confirmed wound healing potential in both animals and humans. Hydroxypropyl methylcellulose (HPMC) and polyethylene oxide (PEO) were included as copolymers. Rheological profiles of spinning solutions containing HPMC, PEO, βG, ethanol and water, were optimized. The nanofiber formation was confirmed by Field Emission Scanning Electron Microscopy (FE-SEM), and both nanofibers with (βG-nanofibers) or without βG (NoβG-nanofibers) were evaluated by their swelling index and FT-IR spectroscopy. The formulations, active ingredient and excipients were tested for their possible in vitro toxicity in keratinocytes. Finally, the wound healing potential of the nanofibers was tested in externally induced excisional wounds in male diabetic db/db mice. Three different doses of βG-nanofibers and the βG-free, NoβG-nanofibers, were evaluated for their in vivo wound healing efficacy. All nanofiber-treatments provided improved wound healing as compared to the negative control (water). All βG-nanofiber treated groups exhibited significantly improved wound healing as compared to the NoβG-nanofiber treated group, indicating the potential of βG-nanofibers as wound dressing.en_US
dc.descriptionAccepted manuscript version, licensed <a href=http://creativecommons.org/licenses/by-nc-nd/4.0/> CC BY-NC-ND 4.0. Published version available at <a href=https://doi.org/10.1016/j.ejps.2018.05.031> https://doi.org/10.1016/j.ejps.2018.05.031</a>.en_US
dc.identifier.citationGrip, J., Engstad, R.E., Skjæveland, I., Škalko-Basnet, N., Isaksson, J.M., Basnet, P. & Holsæter, A.M. (2018). Beta-glucan-loaded nanofiber dressing improves wound healing in diabetic mice. <i>European Journal of Pharmaceutical Sciences, 121</i>, 269-280. https://doi.org/10.1016/j.ejps.2018.05.031en_US
dc.identifier.cristinIDFRIDAID 1589875
dc.identifier.doi10.1016/j.ejps.2018.05.031
dc.identifier.issn0928-0987
dc.identifier.issn1879-0720
dc.identifier.urihttps://hdl.handle.net/10037/14504
dc.language.isoengen_US
dc.publisherElsevieren_US
dc.relation.journalEuropean Journal of Pharmaceutical Sciences
dc.relation.projectIDinfo:eu-repo/grantAgreement/RCN/NAERINGSPH/240123/Norway/Utvikling av nye sårprodukter med SBG som aktiv ingrediens//en_US
dc.rights.accessRightsopenAccessen_US
dc.subjectVDP::Medical disciplines: 700::Basic medical, dental and veterinary science disciplines: 710::Pharmacology: 728en_US
dc.subjectVDP::Medisinske Fag: 700::Basale medisinske, odontologiske og veterinærmedisinske fag: 710::Farmakologi: 728en_US
dc.subjectBeta-glucanen_US
dc.subjectNanofiberen_US
dc.subjectDiabetic db/db miceen_US
dc.subjectNeedle-free electrospinningen_US
dc.subjectWound dressingen_US
dc.subjectWound healingen_US
dc.titleBeta-glucan-loaded nanofiber dressing improves wound healing in diabetic miceen_US
dc.typeJournal articleen_US
dc.typeTidsskriftartikkelen_US
dc.typePeer revieweden_US


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