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dc.contributor.authorTernullo, Selenia
dc.contributor.authorBasnet, Purusotam
dc.contributor.authorHolsæter, Ann Mari
dc.contributor.authorFlaten, Gøril Eide
dc.contributor.authorde Weerd, Louis
dc.contributor.authorSkalko-Basnet, Natasa
dc.date.accessioned2019-02-08T08:35:49Z
dc.date.available2019-02-08T08:35:49Z
dc.date.issued2018-10-07
dc.description.abstractThe topical administration of exogenous human epidermal growth factor (hEGF) is a promising approach for improved chronic wound therapy. To develop therapeutically superior hEGF formulation, we prepared hEGF-containing neutral (NDLs), cationic (CDLs) and anionic (ADLs) deformable liposomes (DLs), respectively, since it is expected that the liposomal surface charge can affect both the liposomal physicochemical properties, their skin penetration potential and therapeutic efficacy of liposome-associated drug. All prepared liposomes were of similar size (300–350 nm) with high hEGF load (~80% entrapment efficacy). Among the studied DLs, ADLs were found to be most promising for sustained release of hEGF, as assessed in vitro using the polyamide membrane. Ex vivo studies revealed that all DLs were excellent systems for skin therapy with hEGF and no penetration of hEGF through the full thickness human skin was detected. ADLs provided a depot exhibiting the highest hEGF retention onto the human skin surface. ADLs also revealed enhanced mitogenic activities in human fibroblasts compared to both NDLs and CDLs after 48 hrs treatment. Moreover, hEGF-containing ADLs significantly enhanced mitogenic activity in fibroblast as compared to activity of hEGF solution (positive control). Similar trends were observed in human keratinocytes after 24 hrs of treatment. We proved that the liposomal surface charge affects the therapeutic potential of hEGF-containing liposomes. hEGF-containing ADLs can be a promising nanosystem-based formulation for localized therapy of chronic wounds.en_US
dc.descriptionAccepted manuscript version, licensed <a href=http://creativecommons.org/licenses/by-nc-nd/4.0/> CC BY-NC-ND 4.0.</a> Published version available at <a href=https://doi.org/10.1016/j.ejps.2018.10.005> https://doi.org/10.1016/j.ejps.2018.10.005</a>.en_US
dc.identifier.citationTernullo, S., Basnet, P., Holsæter, A.M., Flaten, G.E., de Weerd, L. & Skalko-Basnet, N. (2018). Deformable liposomes for skin therapy with human epidermal growth factor: The effect of liposomal surface charge. <i>European Journal of Pharmaceutical Sciences, 125</i>, 163-171. https://doi.org/10.1016/j.ejps.2018.10.005en_US
dc.identifier.cristinIDFRIDAID 1618642
dc.identifier.doi10.1016/j.ejps.2018.10.005
dc.identifier.issn0928-0987
dc.identifier.issn1879-0720
dc.identifier.urihttps://hdl.handle.net/10037/14652
dc.language.isoengen_US
dc.publisherElsevieren_US
dc.relation.journalEuropean Journal of Pharmaceutical Sciences
dc.rights.accessRightsopenAccessen_US
dc.subjectVDP::Medical disciplines: 700::Basic medical, dental and veterinary science disciplines: 710::Pharmacology: 728en_US
dc.subjectVDP::Medisinske Fag: 700::Basale medisinske, odontologiske og veterinærmedisinske fag: 710::Farmakologi: 728en_US
dc.subjecthEGF (human epidermal growth factor)en_US
dc.subjectDeformable liposomesen_US
dc.subjectLiposome surface chargeen_US
dc.subjectHuman skinen_US
dc.subjectChronic wound healingen_US
dc.titleDeformable liposomes for skin therapy with human epidermal growth factor: The effect of liposomal surface chargeen_US
dc.typeJournal articleen_US
dc.typeTidsskriftartikkelen_US
dc.typePeer revieweden_US


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