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dc.contributor.authorIslam, Md Ashraful
dc.contributor.authorUrbarova, Ilona
dc.contributor.authorBruun, Jack-Ansgar
dc.contributor.authorMartinez, Inigo Zubiavrre
dc.date.accessioned2019-03-04T14:20:33Z
dc.date.available2019-03-04T14:20:33Z
dc.date.issued2019-02-20
dc.description.abstractMesenchymal stromal cells (MSCs), given their regenerative potential, are being investigated as a potential therapeutic tool for cartilage lesions. MSCs express several bioactive molecules which act in a paracrine fashion to modulate the tissue microenvironment. Yet, little is known about the divergence of these signalling molecules in different MSC populations. The present study investigated secretomes of stromal cells harvested from Hoffa’s fat pad (HFPSCs), synovial membrane (SMSCs), umbilical cord (UCSCs) and cartilage (ACs) by quantitative liquid chromatography-mass spectrometry (LC-MS/MS) proteomics. Also, multiplex protein arrays and functional assays were performed to compare the constitutive immunomodulatory capabilities of different MSCs. Proteins involved in extracellular matrix degradation and inflammation, such as matrix metalloproteinases (MMPs), interleukin (IL)-17 and complement factors, were downregulated in UCSCs as compared to adult cell sources. Additionally, secretion of transforming growth factor (TGF)-β1 and prostaglandin E2 (PGE2) was enhanced in UCSC supernatants. UCSCs were superior in inhibiting peripheral blood mononuclear cell (PBMC) proliferation, migration and cytokine secretion as compared to adult stromal cells. SMSCs significantly suppressed the proliferation of PBMCs only if they were primed with pro-inflammatory cytokines. Although all cell types repressed human leukocyte antigen-DR isotype (HLADR) surface expression and cytokine release by activated macrophages, only UCSCs significantly blocked IL-6 and IL-12 production. Furthermore, UCSCs supernatants increased aggrecan gene expression in twodimensional chondrocyte cultures. The data demonstrated that UCSCs displayed superior anti-inflammatory and immunosuppressive properties than stromal cells from adult tissues. This allogeneic cell source could potentially be considered as an adjuvant therapy for articular cartilage repair.en_US
dc.description.sponsorshipUiT The Arctic University of Norwayen_US
dc.descriptionSource at <a href=https://doi.org/10.22203/eCM.v037a10 >https://doi.org/10.22203/eCM.v037a10. </a> This article is distributed in accordance with Creative Commons Attribution Licence (<a href=http://creativecommons.org/licenses/by-sa/4.0/>http://creativecommons.org/licenses/by-sa/4.0/</a>).en_US
dc.identifier.citationIslam, A., Urbarova, I., Bruun, J.A. & Martinez-Zubiaurre, I. (2018). Large-scale secretome analyses unveil the superior immunosuppressive phenotype of umbilical cord stromal cells as compared to other adult mesenchymal stromal cells. <i>European Cells and Materials, 37</i>, 153-174. https://doi.org/10.22203/eCM.v037a10en_US
dc.identifier.cristinIDFRIDAID 1679213
dc.identifier.doi10.22203/eCM.v037a10
dc.identifier.issn1473-2262
dc.identifier.urihttps://hdl.handle.net/10037/14821
dc.language.isoengen_US
dc.publisherAO Research Institute Davosen_US
dc.relation.journalEuropean Cells and Materials
dc.rights.accessRightsopenAccessen_US
dc.subjectSecretomeen_US
dc.subjectumbilical cord stromal cellsen_US
dc.subjectchondrocytesen_US
dc.subjectHoffa’s fat pad-derived stromal cellsen_US
dc.subjectsynovial membrane stromal cellsen_US
dc.subjectmesenchymal stromal cellsen_US
dc.subjectimmunomodulationen_US
dc.subjectcartilage repairen_US
dc.subjectVDP::Medical disciplines: 700::Basic medical, dental and veterinary science disciplines: 710en_US
dc.subjectVDP::Medisinske Fag: 700::Basale medisinske, odontologiske og veterinærmedisinske fag: 710en_US
dc.titleLarge-scale secretome analyses unveil the superior immunosuppresive phenotype of umbilical cord stromal cells as compared to other adult mesenchymal stromal cellsen_US
dc.typeJournal articleen_US
dc.typeTidsskriftartikkelen_US
dc.typePeer revieweden_US


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