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dc.contributor.authorDongre, Mitesh
dc.contributor.authorSingh, Bhupender
dc.contributor.authorAung, Kyaw Min
dc.contributor.authorLarsson, Per
dc.contributor.authorMiftakhova, Regina
dc.contributor.authorPersson, Karina
dc.contributor.authorAskarian, Fatemeh
dc.contributor.authorJohannessen, Mona
dc.contributor.authorHofsten, Jonas von
dc.contributor.authorPersson, Jenny L.
dc.contributor.authorErhardt, Marc
dc.contributor.authorTuck, Simon
dc.contributor.authorUhlin, Bernt Eric
dc.contributor.authorWai, Sun Nyunt
dc.date.accessioned2019-03-11T14:17:53Z
dc.date.available2019-03-11T14:17:53Z
dc.date.issued2018-06-07
dc.description.abstractUsing <i>Caenorhabditis elegans</i> as an infection host model for <i>Vibrio cholerae</i> predator interactions, we discovered a bacterial cytotoxin, MakA, whose function as a virulence factor relies on secretion via the flagellum channel in a proton motive force-dependent manner. The MakA protein is expressed from the polycistronic <i>makDCBA</i> (<i>m</i>otility-<i>a</i>ssociated <i>k</i>illing factor) operon. Bacteria expressing <i>makDCBA</i> induced dramatic changes in intestinal morphology leading to a defecation defect, starvation and death in <i>C. elegans</i>. The Mak proteins also promoted <i>V. cholerae</i> colonization of the zebrafish gut causing lethal infection. A structural model of purified MakA at 1.9 Å resolution indicated similarities to members of a superfamily of bacterial toxins with unknown biological roles. Our findings reveal an unrecognized role for <i>V. cholerae</i> flagella in cytotoxin export that may contribute both to environmental spread of the bacteria by promoting survival and proliferation in encounters with predators, and to pathophysiological effects during infections.en_US
dc.description.sponsorshipSwedish Research Councilen_US
dc.descriptionSource at <a href=https://doi.org/10.1038/s42003-018-0065-z>https://doi.org/10.1038/s42003-018-0065-z. </a>en_US
dc.identifier.citationDongre, M., Singh, B. Aung, K.M., Larsson, P., Miftakhova, R., Persson, K. ... Wai, S.N. (2018). Flagella-mediated secretion of a novel <i>Vibrio cholerae</i> cytotoxin affecting both vertebrate and invertebrate hosts. <i>Communications Biology</i>, 1:59. https://doi.org/10.1038/s42003-018-0065-zen_US
dc.identifier.cristinIDFRIDAID 1584490
dc.identifier.doi10.1038/s42003-018-0065-z
dc.identifier.issn2399-3642
dc.identifier.urihttps://hdl.handle.net/10037/14940
dc.language.isoengen_US
dc.publisherNature Researchen_US
dc.relation.journalCommunications Biology
dc.rights.accessRightsopenAccessen_US
dc.subjectBacteriologyen_US
dc.subjectCellular microbiologyen_US
dc.subjectVDP::Medical disciplines: 700::Basic medical, dental and veterinary science disciplines: 710en_US
dc.subjectVDP::Medisinske Fag: 700::Basale medisinske, odontologiske og veterinærmedisinske fag: 710en_US
dc.titleFlagella-mediated secretion of a novel Vibrio cholerae cytotoxin affecting both vertebrate and invertebrate hostsen_US
dc.typeJournal articleen_US
dc.typeTidsskriftartikkelen_US
dc.typePeer revieweden_US


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