Show simple item record

dc.contributor.authorSöllinger, Andrea
dc.contributor.authorTveit, Alexander Tøsdal
dc.contributor.authorPoulsen, Morten
dc.contributor.authorNoel, Samantha Joan
dc.contributor.authorBengtsson, Mia M. B.
dc.contributor.authorBernhardt, Jörg
dc.contributor.authorHellwing, Anne L. Frydendahl
dc.contributor.authorLund, Peter
dc.contributor.authorRiedel, Katharina
dc.contributor.authorSchleper, Christa
dc.contributor.authorOle, Højberg
dc.contributor.authorUrich, Tim
dc.date.accessioned2019-03-12T13:28:21Z
dc.date.available2019-03-12T13:28:21Z
dc.date.issued2018-08-07
dc.description.abstractRuminant livestock is a major source of the potent greenhouse gas methane. The complex rumen microbiome, consisting of bacteria, archaea, and microbial eukaryotes, facilitates anaerobic plant biomass degradation in the cow rumen, leading to methane emissions. Using an integrated approach combining multidomain quantitative metatranscriptomics with gas and volatile fatty acid (VFA) profiling, we aimed at obtaining the most comprehensive picture of the active rumen microbiome during feed degradation to date. Bacterial, archaeal, and eukaryotic biomass, but also methane emissions and VFA concentrations, increased drastically within an hour after feed intake. mRNA profiling revealed a dynamic response of carbohydrate-active enzyme transcripts, transcripts involved in VFA production and methanogenesis. While the relative abundances of functional transcripts did not mirror observed processes, such as methane emissions, transformation to mRNA abundance per gram of rumen fluid echoed ruminant processes. The microbiome composition was highly individual, with, e.g., ciliate, <i>Neocallimastigaceae</i>, <i>Prevotellaceae</i>, <i>Succinivibrionaceae</i>, and <i>Fibrobacteraceae </i>abundances differing between cows. Microbiome individuality was accompanied by inter- and intradomain multifunctional redundancy among microbiome members during feed degradation. This likely enabled the robust performance of the anaerobic degradation process in each rumen. <i>Neocallimastigaceae</i> and ciliates contributed an unexpectedly large share of transcripts for cellulose- and hemicellulose-degrading enzymes, respectively. Methyl-reducing but not CO2-reducing methanogens were positively correlated with methane emissions. While <i>Methanomassiliicoccales</i> switched from methanol to methylamines as electron acceptors, <i>Methanosphaera</i> became the dominating methanol-reducing methanogen. This study for the first time linked rumen meta-omics with processes and enabled holistic insights into the contribution of all microbiome members to feed degradation.<p> <p><i>Importance</i>: Ruminant animals, such as cows, live in a tight symbiotic association with microorganisms, allowing them to feed on otherwise indigestible plant biomass as food sources. Methane is produced as an end product of the anaerobic feed degradation in ruminants and is emitted to the atmosphere, making ruminant animals among the major anthropogenic sources of the potent greenhouse gas methane. Using newly developed quantitative metatranscriptomics for holistic microbiome analysis, we here identified bacterial, archaeal, and eukaryotic key players and the short-term dynamics of the rumen microbiome during anaerobic plant biomass degradation and subsequent methane emissions. These novel insights might pave the way for novel ecologically and economically sustainable methane mitigation strategies, much needed in times of global climate change.en_US
dc.description.sponsorshipUniversity of Vienna OeAD (Austrian Agency for International Mobility and Cooperation in Education, Science and Research) University of Greifswalden_US
dc.descriptionSource at <a href=https://doi.org/10.1128/mSystems.00038-18>https://doi.org/10.1128/mSystems.00038-18. </a>en_US
dc.identifier.citationSöllinger, A., Tveit, A.T., Poulsen, M, Noel, S., Bengtsson, M., Bernhardt, J. ... Urich, T. (2018). Holistic Assessment of Rumen Microbiome Dynamics through Quantitative Metatranscriptomics Reveals Multifunctional Redundancy during Key Steps of Anaerobic Feed Degradation. <i>mSystems, 3</i>(4), e00038-18. https://doi.org/10.1128/mSystems.00038-18en_US
dc.identifier.cristinIDFRIDAID 1649274
dc.identifier.doi10.1128/mSystems.00038-18
dc.identifier.issn2379-5077
dc.identifier.urihttps://hdl.handle.net/10037/14953
dc.language.isoengen_US
dc.publisherAmerican Society for Microbiologyen_US
dc.relation.journalmSystems
dc.rights.accessRightsopenAccessen_US
dc.subjectarchaeaen_US
dc.subjectMethanomassiliicoccalesen_US
dc.subjectcarbohydrate active enzymesen_US
dc.subjectmetatranscriptomicsen_US
dc.subjectmethaneen_US
dc.subjectmethanogenesisen_US
dc.subjectmicrobiomeen_US
dc.subjectrumenen_US
dc.subjectvolatile fatty acidsen_US
dc.subjectVDP::Mathematics and natural science: 400::Zoology and botany: 480en_US
dc.subjectVDP::Matematikk og Naturvitenskap: 400::Zoologiske og botaniske fag: 480en_US
dc.subjectVDP::Mathematics and natural science: 400::Chemistry: 440en_US
dc.titleHolistic Assessment of Rumen Microbiome Dynamics through Quantitative Metatranscriptomics Reveals Multifunctional Redundancy during Key Steps of Anaerobic Feed Degradationen_US
dc.typeJournal articleen_US
dc.typeTidsskriftartikkelen_US
dc.typePeer revieweden_US


File(s) in this item

Thumbnail

This item appears in the following collection(s)

Show simple item record