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dc.contributor.authorBrovold, Henrik
dc.contributor.authorLund, Trine
dc.contributor.authorSvistounov, Dmitri
dc.contributor.authorSolbu, Marit Dahl
dc.contributor.authorJenssen, Trond Geir
dc.contributor.authorYtrehus, Kirsti
dc.contributor.authorZykova, Svetlana
dc.date.accessioned2019-08-02T11:54:07Z
dc.date.available2019-08-02T11:54:07Z
dc.date.issued2019-07-19
dc.description.abstractSeveral epidemiological studies have pointed at serum uric acid (SUA) as an independent risk factor for mortality, diabetes, hypertension, cardiovascular and kidney disease; however, no clear pathogenic pathway is established. Uric acid (UA) crystals show pro-inflammatory properties and can thus create or contribute to the state of chronic low-grade inflammation, a widely accepted pathogenic mechanism in several of the above-mentioned pathologies. On the other hand, soluble uric acid possesses antioxidant properties that might attenuate inflammatory responses. We aimed to explore the net effects of experimentally rising SUA in human whole blood cultures on several mediators of inflammation. production of tNF-α, IL-1ß, IL-1RA, MCP-1 and IL-8 was assessed upon addition of 200 μM UA, 500 μM UA or monosodium urate (MSU) crystals in the presence or absence of 5 ng/ml lipopolysaccharide (LPS). RT-qPCR and multiplex bead based immunoassay were used to measure mRNA expression and cytokine release at 2 and 4 h of culture, respectively. 14C labeled UA was used to assess intracellular uptake of UA. We show that crystallized, but not soluble, UA induces production of pro-inflammatory mediators in human whole blood. Soluble UA is internalized in blood cells but does not potentiate or reduce LPS-induced release of cytokines.en_US
dc.description.sponsorshipUiT – The Arctic University of Norway Tromsø Research Foundationen_US
dc.descriptionSource at <a href=https://doi.org/10.1038/s41598-019-46935-w>https://doi.org/10.1038/s41598-019-46935-w. </a> © The Author(s) 2019en_US
dc.identifier.citationBrovold, H., Lund, T., Svistounov, D., Solbu, M.D., Jenssen, T.G., Ytrehus, K. & Zykova, S.N. (2019). Crystallized but not soluble uric acid elicits pro-inflammatory response in short-term whole blood cultures from healthy men. <i>Scientific Report</i>, 9:10513. https://doi.org/10.1038/s41598-019-46935-wen_US
dc.identifier.cristinIDFRIDAID 1712197
dc.identifier.doi10.1038/s41598-019-46935-w
dc.identifier.urihttps://hdl.handle.net/10037/15829
dc.language.isoengen_US
dc.publisherNature Researchen_US
dc.relation.journalScientific Report
dc.rights.accessRightsopenAccessen_US
dc.subjectVDP::Medical disciplines: 700::Basic medical, dental and veterinary science disciplines: 710en_US
dc.subjectVDP::Medisinske Fag: 700::Basale medisinske, odontologiske og veterinærmedisinske fag: 710en_US
dc.titleCrystallized but not soluble uric acid elicits pro-inflammatory response in short-term whole blood cultures from healthy menen_US
dc.typeJournal articleen_US
dc.typeTidsskriftartikkelen_US
dc.typePeer revieweden_US


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