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dc.contributor.authorChi, Sylvia Ighem
dc.contributor.authorDahl, Mikael
dc.contributor.authorEmblem, Åse
dc.contributor.authorJohansen, Steinar
dc.date.accessioned2019-09-20T11:36:37Z
dc.date.available2019-09-20T11:36:37Z
dc.date.issued2019-06-01
dc.description.abstract<p><i>Background - </i>The mitochondrial genomes of mushroom corals (Corallimorpharia) are remarkable for harboring two complex group I introns; ND5-717 and COI-884. How these autocatalytic RNA elements interfere with mitochondrial RNA processing is currently not known. Here, we report experimental support for unconventional processing events of ND5-717 containing RNA. <p><i>Results - </i>We obtained the complete mitochondrial genome sequences and corresponding mitochondrial transcriptomes of the two distantly related corallimorpharian species <i>Ricordea yuma</i> and <i>Amplexidiscus fenestrafer</i>. All mitochondrial genes were found to be expressed at the RNA-level. Both introns were perfectly removed by autocatalytic splicing, but COI-884 excision appeared more efficient than ND5-717. ND5-717 was organized into giant group I intron elements of 18.1 kb and 19.3 kb in <i>A. fenestrafer</i> and <i>R. yuma</i>, respectively. The intron harbored almost the entire mitochondrial genome embedded within the P8 peripheral segment. <p><i>Conclusion - </i>ND5-717 was removed by group I intron splicing from a small primary transcript that contained a permutated intron–exon arrangement. The splicing pathway involved a circular exon-containing RNA intermediate, which is a hallmark of RNA back-splicing. ND5-717 represents the first reported natural group I intron that becomes excised by back-splicing from a permuted precursor RNA. Back-splicing may explain why Corallimorpharia mitochondrial genomes tolerate giant group I introns.en_US
dc.description.sponsorshipTromsø Research Foundation Research Council of Norway UiT–The Arctic University of Norway Nord Universityen_US
dc.descriptionSource at <a href=https://doi.org/10.1186/s12867-019-0134-y>https://doi.org/10.1186/s12867-019-0134-y</a>.en_US
dc.identifier.citationChi, S.I., Dahl, M., Emblem, Å. & Johansen, S.D. (2019). Giant group I intron in a mitochondrial genome is removed by RNA back-splicing. <i>BMC Molecular Biology, 20</i>, 16. https://doi.org/10.1186/s12867-019-0134-yen_US
dc.identifier.cristinIDFRIDAID 1712777
dc.identifier.doi10.1186/s12867-019-0134-y
dc.identifier.issn1471-2199
dc.identifier.urihttps://hdl.handle.net/10037/16238
dc.language.isoengen_US
dc.publisherSpringer Natureen_US
dc.relation.journalBMC Molecular Biology
dc.rights.accessRightsopenAccessen_US
dc.subjectVDP::Mathematics and natural science: 400::Basic biosciences: 470::Genetics and genomics: 474en_US
dc.subjectVDP::Matematikk og Naturvitenskap: 400::Basale biofag: 470::Genetikk og genomikk: 474en_US
dc.subjectAmplexidiscusen_US
dc.subjectBack-splicingen_US
dc.subjectCatalytic RNAen_US
dc.subjectGroup I intronen_US
dc.subjectIntron retentionen_US
dc.subjectMitochondrial RNAen_US
dc.subjectRibozymeen_US
dc.subjectRicordeaen_US
dc.titleGiant group I intron in a mitochondrial genome is removed by RNA back-splicingen_US
dc.typeJournal articleen_US
dc.typeTidsskriftartikkelen_US
dc.typePeer revieweden_US


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