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dc.contributor.authorJøraholmen, May Wenche
dc.contributor.authorBasnet, Purusotam
dc.contributor.authorTostrup, Mia
dc.contributor.authorMoueffaq, Sabrin
dc.contributor.authorSkalko-Basnet, Natasa
dc.date.accessioned2019-10-03T11:59:24Z
dc.date.available2019-10-03T11:59:24Z
dc.date.issued2019-01-27
dc.description.abstractNatural polyphenols, such as resveratrol (RES) or epicatechin (EPI), are attractive for treatments of various diseases, including vaginal infections and inflammation, because of their strong anti-oxidative and anti-inflammatory properties. However, their low solubility and consequent poor bioavailability limit their therapeutic uses. To overcome these limitations, a vaginal delivery system comprising either RES or EPI liposomes-in-hydrogel was developed. This system permits therapeutic action of both liposomal polyphenol (RES or EPI) and chitosan-based hydrogel. Liposomes of around 200 nm and entrapment efficiency of 81% and 77% for RES and EPI, respectively, were incorporated into chitosan hydrogel, respectively. Medium molecular weight chitosan (2.5%, w/w) was found to have optimal texture properties and mucoadhesiveness in ex vivo conditions. The in vitro release studies confirmed the sustained release of polyphenols from the system. Both liposomal polyphenols and polyphenols-in-liposomes-in-hydrogel exhibited only minor effects on cell toxicity. EPI showed superior radical scavenging activity at lower concentrations compared to antioxidants vitamin C and E. Anti-inflammatory activity expressed as the inhibitory activity of formulations on the NO production in the LPS-induced macrophages (RAW 264.7) confirmed the superiority of EPI liposomes-in-hydrogel. The plain liposomes-in-hydrogel also exhibited potent anti-inflammatory activity, suggesting that chitosan hydrogel acts in synergy regarding anti-inflammatory effect of formulation.en_US
dc.description.sponsorshipNorthern Norway Regional Health Authorityen_US
dc.descriptionSource at <a href=https://doi.org/10.3390/pharmaceutics11020053>https://doi.org/10.3390/pharmaceutics11020053</a>.en_US
dc.identifier.citationJøraholmen, M.W., Basnet, P., Tostrup, M.J., Moueffaq, S. & Škalko-Basnet, N. (2019). Localized Therapy of Vaginal Infections and Inflammation: Liposomes-In-Hydrogel Delivery System for Polyphenols. <i>Pharmaceutics, 11</i>(2), 53. https://doi.org/10.3390/pharmaceutics11020053en_US
dc.identifier.cristinIDFRIDAID 1666268
dc.identifier.doi10.3390/pharmaceutics11020053
dc.identifier.issn1999-4923
dc.identifier.urihttps://hdl.handle.net/10037/16321
dc.language.isoengen_US
dc.publisherMDPIen_US
dc.relation.journalPharmaceutics
dc.rights.accessRightsopenAccessen_US
dc.subjectVDP::Medical disciplines: 700::Clinical medical disciplines: 750::Gynecology and obstetrics: 756en_US
dc.subjectVDP::Medisinske Fag: 700::Klinisk medisinske fag: 750::Gynekologi og obstetrikk: 756en_US
dc.subjectpolyphenolsen_US
dc.subjectresveratrolen_US
dc.subjectepicatechinen_US
dc.subjectliposomesen_US
dc.subjecthydrogelen_US
dc.subjectchitosanen_US
dc.subjectvaginal drug deliveryen_US
dc.titleLocalized Therapy of Vaginal Infections and Inflammation: Liposomes-In-Hydrogel Delivery System for Polyphenolsen_US
dc.typeJournal articleen_US
dc.typeTidsskriftartikkelen_US
dc.typePeer revieweden_US


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