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dc.contributor.authorSales, Naiara Guimaraes
dc.contributor.authorWangensteen Fuentes, Owen Simon
dc.contributor.authorCarvalho, Daniel C.
dc.contributor.authorMariani, Stefano
dc.date.accessioned2019-11-01T14:34:35Z
dc.date.available2019-11-01T14:34:35Z
dc.date.issued2019-05-31
dc.description.abstractEnvironmental DNA (eDNA) has rapidly emerged as a promising biodiversity monitoring technique, proving to be a sensitive and cost‐effective method for species detection. Despite the increasing popularity of eDNA, several questions regarding its limitations remain to be addressed. We investigated the effect of sampling medium and time, and preservation methods, on fish detection performance based on eDNA metabarcoding of neotropical freshwater samples. Water and sediment samples were collected from 11 sites along the Jequitinhonha River, Southeastern Brazil; sediment samples were stored in ethanol, while the same amounts of water per sample (3 L) were stored in a cool box with ice, as well as by adding the cationic surfactant benzalkonium chloride (BAC). Sediment and water samples yielded a similar amount of fish MOTUs (237 vs. 239 in the first sampling event, and 153 vs. 142 in the second sampling event). Water stored in ice provided better results than those preserved in BAC (239 and 142 vs. 194 and 71 MOTUs). While documenting the effectiveness of eDNA surveys as practical tools for fish biodiversity monitoring in poorly accessible areas, we showed that keeping water samples cooled results in greater eDNA recovery and taxon detection than by adding cationic surfactants (BAC) as sample preservatives. Furthermore, by comparing two sets of samples collected from the same locations at a 3‐week interval, we highlight the importance of conducting multiple sampling events when attempting to recover a realistic picture of fish assemblages in lotic systems.en_US
dc.descriptionSource at <a href=https://doi.org/10.1002/edn3.14>https://doi.org/10.1002/edn3.14. </a>© 2019 The Authorsen_US
dc.identifier.citationSales, N.G., Wangensteen, O.S., Carvalho, D.C. & Mariani, S. (2019). Influence of preservation methods, sample medium and sampling time on eDNA recovery in a neotropical river. <i>Environmental DNA, 1</i>(2), 119-130. https://doi.org/10.1002/edn3.14en_US
dc.identifier.cristinIDFRIDAID 1742831
dc.identifier.doi10.1002/edn3.14
dc.identifier.issn2637-4943
dc.identifier.urihttps://hdl.handle.net/10037/16574
dc.language.isoengen_US
dc.publisherWileyen_US
dc.relation.journalEnvironmental DNA
dc.rights.accessRightsopenAccessen_US
dc.subjectVDP::Agriculture and fishery disciplines: 900::Fisheries science: 920en_US
dc.subjectVDP::Landbruks- og Fiskerifag: 900::Fiskerifag: 920en_US
dc.titleInfluence of preservation methods, sample medium and sampling time on eDNA recovery in a neotropical riveren_US
dc.typeJournal articleen_US
dc.typeTidsskriftartikkelen_US
dc.typePeer revieweden_US


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