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dc.contributor.authorErkinharju, Toni
dc.contributor.authorStrandskog, Guro
dc.contributor.authorVågnes, Øyvind
dc.contributor.authorHordvik, Ivar
dc.contributor.authorDalmo, Roy Ambli
dc.contributor.authorSeternes, Tore
dc.date.accessioned2019-11-08T12:58:27Z
dc.date.available2019-11-08T12:58:27Z
dc.date.issued2019-10-20
dc.description.abstractAtlantic lumpfish were vaccinated by intramuscular (im) or intraperitoneal (ip) injection with a multivalent oil‐based vaccine, while control fish were injected with phosphate‐buffered saline. Four lumpfish per group were sampled for skin/muscle and head kidney tissue at 0, 2, 7, 21 and 42 days post‐immunization (dpi) for histopathology and immunohistochemistry (IHC). Gene expressions of secretory IgM, membrane‐bound IgM, IgD, TCRα, CD3ε and MHC class IIβ were studied in tissues by using qPCR. Im. vaccinated fish showed vaccine‐induced inflammation with formation of granulomas and increasing number of eosinophilic granulocyte‐like cells over time. On IHC sections, we observed diffuse intercellular staining of secretory IgM at the injection site at 2 dpi, while IgM + cells appeared in small numbers at 21 and 42 dpi. Skin/muscle samples from im. vaccinated fish demonstrated an increase in gene expression of IgM mRNA (secretory and membrane‐bound) at 21 and 42 dpi and small changes for other genes. Our results indicated that im. vaccination of lumpfish induced local IgM production at the vaccine injection site, with no apparent proliferation of IgM + cells. Eosinophilic granulocyte‐like cells appeared shortly after im. injection and increased in numbers as the inflammation progressed.en_US
dc.descriptionSource at <a href=https://doi.org/10.1111/jfd.13101>https://doi.org/10.1111/jfd.13101. </a> © 2019 The Authorsen_US
dc.identifier.citationErkinharju, T., Strandskog, G., Vågnes, Ø., Hordvik, I., Dalmo, R.A. & Seternes, T. (2019). Intramuscular vaccination of Atlantic lumpfish (<i>Cyclopterus lumpus</i> L.) induces inflammatory reactions and local immunoglobulin M production at the vaccine administration site. <i>Journal of Fish Diseases</i>. https://doi.org/10.1111/jfd.13101en_US
dc.identifier.cristinIDFRIDAID 1744813
dc.identifier.doihttps://doi.org/10.1111/jfd.13101
dc.identifier.issn0140-7775
dc.identifier.issn1365-2761
dc.identifier.urihttps://hdl.handle.net/10037/16629
dc.language.isoengen_US
dc.publisherWileyen_US
dc.relation.journalJournal of Fish Diseases
dc.relation.projectIDinfo:eu-repo/grantAgreement/RCN/HAVBRUK2/244396/Norway/Basic immunology studies and development of tools to monitor immune responses in cleaner fish//en_US
dc.relation.projectIDinfo:eu-repo/grantAgreement/RCN/HAVBRUK2/280847/Norway/Comparing the protection of attenuated and inactivated virus vaccines against pancreas disease and heart and skeletal muscle inflammation//en_US
dc.relation.projectIDinfo:eu-repo/grantAgreement/RCN/NANO2021/239140/Norway/Nanoparticle encapsulation of plant-based vaccine against piscine reovirus infection in salmon//en_US
dc.rights.accessRightsopenAccessen_US
dc.subjectVDP::Agriculture and fishery disciplines: 900::Fisheries science: 920en_US
dc.subjectVDP::Landbruks- og Fiskerifag: 900::Fiskerifag: 920en_US
dc.titleIntramuscular vaccination of Atlantic lumpfish (Cyclopterus lumpus L.) induces inflammatory reactions and local immunoglobulin M production at the vaccine administration siteen_US
dc.typeJournal articleen_US
dc.typeTidsskriftartikkelen_US
dc.typePeer revieweden_US


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