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dc.contributor.authorJoseph, Joby
dc.contributor.authorFaiz, Kandankulangara P.
dc.contributor.authorLahrberg, Marcel
dc.contributor.authorTinguely, Jean-Claude
dc.contributor.authorAhluwalia, Balpreet Singh
dc.date.accessioned2019-12-19T13:23:39Z
dc.date.available2019-12-19T13:23:39Z
dc.date.issued2019-11-18
dc.description.abstractApplying structured instead of plane illumination in widefield optical fluorescence microscopy can improve the spatial resolution beyond what is known as the Abbe limit. In general it is not only the resolution of an imaging system that is of interest but also its field of view (FOV). These two parameters are expressed in the space-bandwidth product (SBP). Here we introduce a modified structured illumination microscopy (SIM) approach that offers a larger SBP than any other available implementation. This is achieved through a transillumination geometry instead of the typical epifluorescent configuration. Compared to conventional SIM, the illumination path is decoupled from the objective lens by using a multi-mirror setup to generate the sinusoidal interference pattern for structured illumination in transmission mode. The spatial frequency of the illumination pattern can be controlled by changing the angle of the mirrors, achieving comparably fine patterns over a large FOV. In this work simulation results demonstrate the potential resolution improvement to be expected by the suggested implementation. Preliminary experimental results demonstrate phase-shifting ability and the stability of fringe frequencies over a large FOV of (&asymp;16 mm<sup>2</sup>) at different numerical apertures, fulfilling the prerequisites for SIM acquisition.en_US
dc.identifier.citationJoseph J, Faiz, Lahrberg M, Tinguely J, Ahluwalia BS. Improving the space-bandwidth product of structured illumination microscopy using a transillumination configuration. Journal of Physics D: Applied Physics. 2019(53)en_US
dc.identifier.cristinIDFRIDAID 1755356
dc.identifier.doihttps://doi.org/10.1088/1361-6463/ab4e68
dc.identifier.issn0022-3727
dc.identifier.issn1361-6463
dc.identifier.urihttps://hdl.handle.net/10037/16968
dc.language.isoengen_US
dc.publisherIOP Publishing Ltden_US
dc.relation.ispartofLahrberg, M. (2019). Novel strategies for super-resolution fluorescence microscopy. (Doctoral thesis). <a href=https://hdl.handle.net/10037/17093>https://hdl.handle.net/10037/17093</a>.
dc.relation.journalJournal of Physics D: Applied Physics
dc.rights.accessRightsopenAccessen_US
dc.rights.holderCopyright 2019 The Author(s)en_US
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.rightsAttribution 4.0 International*
dc.subjectVDP::Mathematics and natural science: 400::Physics: 430::Electromagnetism, acoustics, optics: 434en_US
dc.subjectVDP::Matematikk og Naturvitenskap: 400::Fysikk: 430::Elektromagnetisme, akustikk, optikk: 434en_US
dc.titleImproving the space-bandwidth product of structured illumination microscopy using a transillumination configurationen_US
dc.type.versionpublishedVersionen_US
dc.typeJournal articleen_US
dc.typeTidsskriftartikkelen_US
dc.typePeer revieweden_US


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Attribution 4.0 International
Except where otherwise noted, this item's license is described as Attribution 4.0 International