Show simple item record

dc.contributor.authorGarcés-Pastor, Sandra
dc.contributor.authorWangensteen Fuentes, Owen Simon
dc.contributor.authorPérez-Haase, Aaron
dc.contributor.authorPèlachs, Albert
dc.contributor.authorPérez-Obiol, Ramon
dc.contributor.authorCañellas-Boltà, Nuria
dc.contributor.authorMariani, Stefano
dc.contributor.authorVegas-Villarubia, Teresa
dc.date.accessioned2019-12-23T12:59:43Z
dc.date.available2019-12-23T12:59:43Z
dc.date.issued2019-09-30
dc.description.abstractPeat bogs located in high mountains are suitable places to study local environmental responses to climate variability. These ecosystems host a large number of eukaryotes with diverse taxonomic and functional diversity. We carried out a metabarcoding study using universal 18S and COI markers to explore the composition of past and present eukaryotic communities of a Pyrenean peat bog ecosystem. We assessed the molecular biodiversity of four different moss micro-habitats along a flood gradient in the lentic Bassa Nera system (Central Pyrenees). Five samples collected from different sediment depths at the same study site were also analysed, to test the suitability of these universal markers for studying paleoecological communities recovered from ancient DNA and to compare the detected DNA sequences to those obtained from the modern community. We also compared the information provided by the sedimentary DNA to the reconstruction from environmental proxies such as pollen and macro-remains from the same record. We successfully amplified ancient DNA with both universal markers from all sediment samples, including the deepest one (~ 10,000 years old). Most of the metabarcoding reads obtained from sediment samples, however, were assigned to living edaphic organisms and only a small fraction of those reads was considered to be derived from paleoecological communities. Inferences from ancient sedimentary DNA were complementary to the reconstruction based on pollen and macro-remains, and the combined records reveal more detailed information. This molecular study yielded promising findings regarding the diversity of modern eukaryotic peat bog communities. Nevertheless, even though information about past communities could be retrieved from sediment samples, preferential amplification of DNA from living communities is a caveat for the use of universal metabarcoding markers in paleoecology.en_US
dc.descriptionThis is a post-peer-review, pre-copyedit version of an article published in the <i>Journal of Paleolimnology</i>. The final authenticated version is available online at <a href=https://doi.org/10.1007/s10933-019-00097-x>https://doi.org/10.1007/s10933-019-00097-x</a>.en_US
dc.identifier.citationGarcés-Pastor S, Wangensteen Fuentes OS, Pérez-Haase A, Pèlachs A, Pérez-Obiol R, Cañellas-Boltà N, Mariani S, Vegas-Villarubia. DNA metabarcoding reveals modern and past eukaryotic communities in a high-mountain peat bog system. Journal of Paleolimnology. 2019;62:425-441en_US
dc.identifier.cristinIDFRIDAID 1759543
dc.identifier.doi10.1007/s10933-019-00097-x
dc.identifier.issn0921-2728
dc.identifier.issn1573-0417
dc.identifier.urihttps://hdl.handle.net/10037/16985
dc.language.isoengen_US
dc.publisherSpringer Natureen_US
dc.relation.journalJournal of Paleolimnology
dc.rights.accessRightsopenAccessen_US
dc.rights.holder© Springer Nature B.V. 2019en_US
dc.subjectVDP::Mathematics and natural science: 400::Basic biosciences: 470::Genetics and genomics: 474en_US
dc.subjectVDP::Matematikk og Naturvitenskap: 400::Basale biofag: 470::Genetikk og genomikk: 474en_US
dc.titleDNA metabarcoding reveals modern and past eukaryotic communities in a high-mountain peat bog systemen_US
dc.type.versionacceptedVersionen_US
dc.typeJournal articleen_US
dc.typeTidsskriftartikkelen_US
dc.typePeer revieweden_US


File(s) in this item

Thumbnail

This item appears in the following collection(s)

Show simple item record