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dc.contributor.authorReine, Trine M.
dc.contributor.authorLanzalaco, Francesca
dc.contributor.authorKristiansen, Oddrun
dc.contributor.authorEnget, Anne Randi
dc.contributor.authorSatchell, Simon
dc.contributor.authorJenssen, Trond Geir
dc.contributor.authorKolset, Svein Olav
dc.date.accessioned2020-01-16T21:47:20Z
dc.date.available2020-01-16T21:47:20Z
dc.date.issued2019-01-31
dc.description.abstract<p><i>Background - </i>Diabetic nephropathy is the most common cause of end‐stage renal failure in the western world and Asia. The mechanisms are not fully elucidated, but disruption of glomerular endothelial glycocalyx and shedding of its components including syndecans has been implicated. <p><i>Aims - </i>We hypothesize that reduced glomerular filtration in diabetes is caused by disruption of endothelial glycocalyx in glomeruli, including increased shedding of syndecan‐4. The aim of this study was to determine the effects of experimental diabetic conditions by means of hyperglycemia and IL‐1β exposure on syndecan‐4 shedding in GEnC, and to investigate regulation of shedding by sheddases. <p><i>Results - </i>We found that in GEnC the expression of syndecan‐4 is higher than that of the other syndecans. In polarized GEnC, apical shedding of syndecan‐4 and syndecan‐4 gene expression was increased by 60% after IL‐1β‐stimulation, but not affected by hyperglycemic conditions. This was accompanied by a 50% increase in MMP9 gene expression in IL‐1β‐stimulated cells but not hyperglycemia. MMP9 knockdown reduced syndecan‐4 shedding by 50%. <p><i>Conclusion - </i>IL‐1β but not hyperglycemia increases the shedding of syndecan‐4 from GEnC in an MMP9‐dependent manner. This provides a potential mechanism of GEnC damage in diabetes and other inflammatory conditions.en_US
dc.descriptionThis is the peer reviewed version of the following article: Reine, T.M., Lanzalaco, F., Kristiansen, O., Alvestad, A.R., Satchell, S., Jenssen, T.G. & Kolset, S.O. (2019). Matrix metalloproteinase-9 mediated shedding of syndecan-4 in glomerular endothelial cells. <i>Microcirculation, 26</i>(4), e12534, which has been published in final form at <a href=https://doi.org/10.1111/micc.12534>https://doi.org/10.1111/micc.12534</a>. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Use of Self-Archived Versions.en_US
dc.identifier.citationReine, T.M., Lanzalaco, F., Kristiansen, O., Alvestad, A.R., Satchell, S., Jenssen, T.G. & Kolset, S.O. (2019). Matrix metalloproteinase-9 mediated shedding of syndecan-4 in glomerular endothelial cells. <i>Microcirculation, 26</i>(4), e12534. https://doi.org/10.1111/micc.12534en_US
dc.identifier.cristinIDFRIDAID 1692062
dc.identifier.doi10.1111/micc.12534
dc.identifier.issn1073-9688
dc.identifier.issn1549-8719
dc.identifier.urihttps://hdl.handle.net/10037/17125
dc.language.isoengen_US
dc.publisherWileyen_US
dc.relation.journalMicrocirculation
dc.rights.accessRightsopenAccessen_US
dc.rights.holder© 2019 John Wiley & Sons Ltden_US
dc.subjectVDP::Medical disciplines: 700::Clinical medical disciplines: 750::Nephrology, urology: 772en_US
dc.subjectVDP::Medisinske Fag: 700::Klinisk medisinske fag: 750::Nefrologi, urologi: 772en_US
dc.titleMatrix metalloproteinase-9 mediated shedding of syndecan-4 in glomerular endothelial cellsen_US
dc.type.versionacceptedVersionen_US
dc.typeJournal articleen_US
dc.typeTidsskriftartikkelen_US
dc.typePeer revieweden_US


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