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dc.contributor.authorDrejer, Eivind Bøe
dc.contributor.authorChan, Dennis
dc.contributor.authorHaupka, Carsten
dc.contributor.authorWendisch, Volker F.
dc.contributor.authorBrautaset, Trygve
dc.contributor.authorIrla, Marta Katarzyna
dc.date.accessioned2020-01-24T09:55:15Z
dc.date.available2020-01-24T09:55:15Z
dc.date.issued2019-12-10
dc.description.abstractMethanol is an attractive alternative non-food feedstock for industrial fermentations that can be used instead of sugar-based raw materials. Here, the thermophilic and methylotrophic bacterium <i>Bacillus methanolicus</i> MGA3 was metabolically engineered to produce the platform chemical (<i>R</i>)-acetoin from methanol at 50 °C. Three different heterologous <i>alsSD/budAB</i> operons, each encoding acetolactate synthase and acetolactate decarboxylase, were functionally expressed under control of an inducible promoter in <i>B. methanolicus</i> MGA3, resulting in up to 0.26 ± 0.04 g L<sup>−1</sup> of (<i>R</i>)-acetoin titer in shake flask cultivations. To further improve acetoin production, a total of six different genes or operons were expressed in the acetoin producing strains to increase supply of the acetoin precursor pyruvate. In particular, expression of a gene coding for malic enzyme from <i>Geobacillus stearothermophilus</i> in combination with the isocitrate lyase gene from <i>B. methanolicus</i> MGA3 increased acetoin titers 1.6-fold up to 0.42 ± 0.01 g L<sup>−1</sup> which corresponds to 0.07 g g<sup>−1</sup> methanol. This resulted in an MGA3 strain overproducing 4 recombinant enzymes in total from two different plasmids with two distinct antibiotics resistance markers, demonstrating the increased complexity of metabolic engineering allowed by newly developed genetic tools for this organism. To our knowledge, this is the first demonstration of microbial production of acetoin from methanol.en_US
dc.identifier.citationDrejer EB, Chan D, Haupka, Wendisch VF, Brautaset T, Irla MK. Methanol-based acetoin production by genetically engineered Bacillus methanolicus. Green Chemistry. 2019en_US
dc.identifier.cristinIDFRIDAID 1762688
dc.identifier.doi10.1039/c9gc03950c
dc.identifier.issn1463-9262
dc.identifier.issn1463-9270
dc.identifier.urihttps://hdl.handle.net/10037/17214
dc.language.isoengen_US
dc.publisherRoyal Society of Chemistryen_US
dc.relation.journalGreen Chemistry
dc.rights.accessRightsopenAccessen_US
dc.rights.holder© The Royal Society of Chemistry 2020en_US
dc.subjectVDP::Mathematics and natural science: 400en_US
dc.subjectVDP::Matematikk og Naturvitenskap: 400en_US
dc.titleMethanol-based acetoin production by genetically engineered Bacillus methanolicusen_US
dc.type.versionacceptedVersionen_US
dc.typeJournal articleen_US
dc.typeTidsskriftartikkelen_US
dc.typePeer revieweden_US


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