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dc.contributor.authorHäussler, Ragna S.
dc.contributor.authorBendes, Annika
dc.contributor.authorIglesias, Maria Jesus
dc.contributor.authorSanchez-Rivera, Laura
dc.contributor.authorDodig‐Crnković, Tea
dc.contributor.authorByström, Sanna
dc.contributor.authorFredolini, Claudia
dc.contributor.authorBirgersson, Elin
dc.contributor.authorDale, Matilda
dc.contributor.authorEdfors, Fredrik
dc.contributor.authorFagerberg, Linn
dc.contributor.authorRockberg, Johan
dc.contributor.authorTegel, Hanna
dc.contributor.authorUhlén, Mathias
dc.contributor.authorQundos, Ulrika
dc.contributor.authorSchwenk, Jochen M.
dc.date.accessioned2020-02-11T12:53:09Z
dc.date.available2020-02-11T12:53:09Z
dc.date.issued2019-07-06
dc.description.abstractThe plasma proteome offers a clinically useful window into human health. Recent advances from highly multiplexed assays now call for appropriate pipelines to validate individual candidates. Here, a workflow is developed to build dual binder sandwich immunoassays (SIA) and for proteins predicted to be secreted into plasma. Utilizing suspension bead arrays, ≈1800 unique antibody pairs are first screened against 209 proteins with recombinant proteins as well as EDTA plasma. Employing 624 unique antibodies, dilution‐dependent curves in plasma and concentration‐dependent curves of full‐length proteins for 102 (49%) of the targets are obtained. For 22 protein assays, the longitudinal, interindividual, and technical performance is determined in a set of plasma samples collected from 18 healthy subjects every third month over 1 year. Finally, 14 of these assays are compared with with SIAs composed of other binders, proximity extension assays, and affinity‐free targeted mass spectrometry. The workflow provides a multiplexed approach to screen for SIA pairs that suggests using at least three antibodies per target. This design is applicable for a wider range of targets of the plasma proteome, and the assays can be applied for discovery but also to validate emerging candidates derived from other platforms.en_US
dc.identifier.citationHäussler, Bendes, Iglesias, Sanchez-Rivera, Dodig‐Crnković, Byström, Fredolini, Birgersson, Dale, Edfors, Fagerberg, Rockberg, Tegel, Uhlén M, Qundos, Schwenk. Systematic development of sandwich immunoassays for the plasma secretome. Proteomics. 2019;19:1900008(15):1-19en_US
dc.identifier.cristinIDFRIDAID 1722522
dc.identifier.doi10.1002/pmic.201900008
dc.identifier.issn1615-9853
dc.identifier.issn1615-9861
dc.identifier.urihttps://hdl.handle.net/10037/17367
dc.language.isoengen_US
dc.publisherWileyen_US
dc.relation.journalProteomics
dc.rights.accessRightsopenAccessen_US
dc.rights.holderCopyright 2019 The Author(s)en_US
dc.subjectVDP::Medical disciplines: 700::Basic medical, dental and veterinary science disciplines: 710en_US
dc.subjectVDP::Medisinske Fag: 700::Basale medisinske, odontologiske og veterinærmedisinske fag: 710en_US
dc.titleSystematic development of sandwich immunoassays for the plasma secretomeen_US
dc.type.versionpublishedVersionen_US
dc.typeJournal articleen_US
dc.typeTidsskriftartikkelen_US
dc.typePeer revieweden_US


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