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dc.contributor.authorHaug, Bjørn Helge
dc.contributor.authorHald, Øyvind
dc.contributor.authorUtnes, Peter
dc.contributor.authorRoth, Sarah A
dc.contributor.authorLøkke, Cecilie
dc.contributor.authorFlægstad, Trond
dc.contributor.authorEinvik, Christer
dc.date.accessioned2020-05-13T07:57:24Z
dc.date.available2020-05-13T07:57:24Z
dc.date.issued2015
dc.description.abstract<p><i>Background:</i> In recent years, evidence has accumulated indicating that both normal and cancer cells communicate via the release and delivery of macromolecules packed into extracellular membrane vesicles. <p><i>Materials and Methods:</i> We isolated nano-sized extracellular vesicles from MYCN-amplified neuroblastoma cell lines using ultracentrifugation and exosome precipitation (Exoquick) protocols. These vesicles were characterized by transmission electron microscopy (TEM), nanoparticle tracking analysis and western blotting. Exosomal miRNA profiles were obtained using a reverse transcription-polymerase chain reaction (RT-PCR) ready-to-use panel measuring a total of 742 miRNAs. <p><i>Results:</i> In this study, we showed that MYCN-amplified neuroblastoma cell lines secrete populations of miRNAs inside small exosome-like vesicular particles. These particles were shown to be taken-up by recipient cells. By profiling the miRNA content, we demonstrated high expression of a group of established oncomirs in exosomes from two MYCN-amplified neuroblastoma cell lines. Despite the fact that other studies have demonstrated the ability of exosomal miRNAs both to repress mRNA targets and to stimulate Toll-like receptor-8 (TLR8) signaling in recipient cells, we did not observe these effects with exosomes from MYCN-amplified neuroblastoma cells. However, functional enrichment analysis reveals that mRNA targets of highly expressed exosomal miRNAs are associated with a range of cellular and molecular functions related to cell growth and cell death. <p><i>Conclusion:</i> MYCN-amplified neuroblastoma cell lines secrete exosome-like particles containing oncogenic miRNAs. This work showed for the first time that neuroblastoma cells secrete exosome-like particles containing miRNAs with potential roles in cancer progression. These findings indicate a new way for MYCN-amplified neuroblastoma cells to interact with the tumor environment.en_US
dc.descriptionSource at <a href=http://ar.iiarjournals.org/content/35/5/2521.abstract>http://ar.iiarjournals.org/content/35/5/2521.abstract</a>.en_US
dc.identifier.citationHaug BH, Hald Ø, Utnes PA, Roth R, Løkke C, Flægstad T, Einvik C. Exosome-like extracellular vesicles from MYCN-amplified neuroblastoma cells contain oncogenic miRNAs. Anticancer Research. 2015;35(5):2521-2530en_US
dc.identifier.cristinIDFRIDAID 1256678
dc.identifier.issn0250-7005
dc.identifier.issn1791-7530
dc.identifier.urihttps://hdl.handle.net/10037/18269
dc.language.isoengen_US
dc.publisherInternational Institute of Anticancer Researchen_US
dc.relation.ispartofHald, Ø.H. (2020). Molecular aspects of high-risk neuroblastoma and novel therapeutic opportunities. (Doctoral thesis). <a href=https://hdl.handle.net/10037/18271>https://hdl.handle.net/10037/18271</a>.
dc.relation.journalAnticancer Research
dc.rights.accessRightsopenAccessen_US
dc.rights.holderCopyright 2015 International Institute of Anticancer Researchen_US
dc.subjectVDP::Medical disciplines: 700::Clinical medical disciplines: 750::Oncology: 762en_US
dc.subjectVDP::Medisinske Fag: 700::Klinisk medisinske fag: 750::Onkologi: 762en_US
dc.titleExosome-like extracellular vesicles from MYCN-amplified neuroblastoma cells contain oncogenic miRNAsen_US
dc.type.versionpublishedVersionen_US
dc.typeJournal articleen_US
dc.typeTidsskriftartikkelen_US
dc.typePeer revieweden_US


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