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dc.contributor.authorSlettjord, Tiril Hoffstrøm
dc.contributor.authorSekkenes, Hege J.
dc.contributor.authorChi, Heng
dc.contributor.authorBøgwald, Jarl
dc.contributor.authorSwain, Trilochan
dc.contributor.authorDalmo, Roy Ambli
dc.contributor.authorSwain, Jaya Kumari
dc.date.accessioned2020-06-17T11:17:09Z
dc.date.available2020-06-17T11:17:09Z
dc.date.issued2020-04-20
dc.description.abstractThe overexpression of <i>GATA-3, T-bet</i> and <i>TGF-ß</i> may theoretically induce <i>IL-4/A, IFN-γ</i> and <i>IL-17A</i> expression, respectively. Whether this also applies to fish is not yet known. The plasmid vectors encoding reporter gene (RFP)-tagged <i>T-bet, GATA-3</i> and <i>TGF-ß</i> were used as overexpression tools, transfected into cells or injected intramuscularly to monitor the expression of <i>IFN-γ, IL-4/13A</i> and <i>IL-17A</i>. In addition, the fish were either experimentally challenged with <i>Vibrio anguillarum</i> (VA group) or <i>Piscirickettsia salmonis</i> (PS group). The reporter gene (RFP) inserted upstream of the <i>GATA-3, T-bet</i> and <i>TGF-ß</i> genes, was observed in muscle cell nuclei and in inflammatory cells after intramuscular (i.m.) injection. PS group: following the injection of <i>GATA-3</i> and <i>T-bet</i>-encoding plasmids, the expression of <i>GATA-3</i> and <i>T-bet</i> was high at the injection site. The spleen expression of <i>IFN-γ</i>, following the injection of a T-bet-encoding plasmid, was significantly higher on day 2. VA group: The <i>T-bet</i> and <i>GATA-3</i>-overexpressing fish expressed high <i>T-bet</i> and <i>GATA-3</i> mRNA levels in the muscles and on day 4 post-challenge. The expression of <i>TGF-ß</i> in the muscles of fish injected with <i>TGF-ß</i>-encoding plasmids was significantly higher on days 7 (8 days pre-challenge) and 19 (4 days after challenge). The protective effects of the overexpression of <i>T-bet, GATA-3</i> and <i>TGF-ß</i> on both bacterial infections were negligible.en_US
dc.identifier.citationSlettjord TH, Sekkenes, Chi H, Bøgwald J, Swain T, Dalmo RA, Swain K. Overexpression of T-bet, GATA-3 and TGF-ß induces IFN-γ, IL-4/13A, and IL-17A expression in Atlantic salmon. Biology (Basel). 2020;9:82(4):1-17en_US
dc.identifier.cristinIDFRIDAID 1812645
dc.identifier.doi10.3390/biology9040082
dc.identifier.issn2079-7737
dc.identifier.urihttps://hdl.handle.net/10037/18586
dc.language.isoengen_US
dc.publisherMDPIen_US
dc.relation.journalBiology (Basel)
dc.relation.projectIDinfo:eu-repo/grantAgreement/RCN/NANO2021/239140/Norway/Nanoparticle encapsulation of plant-based vaccine against piscine reovirus infection in salmon//en_US
dc.relation.projectIDinfo:eu-repo/grantAgreement/RCN/HAVBRUK2/237315/Norway/Fish Virus Vaccines/ViVaFish/en_US
dc.relation.projectIDinfo:eu-repo/grantAgreement/RCN/HAVBRUKS/183204/Norway/Indo-Norwegian platform on fish and shellfish vaccine development/VaccInAqua/en_US
dc.rights.accessRightsopenAccessen_US
dc.rights.holderCopyright 2020 The Author(s)en_US
dc.subjectVDP::Agriculture and fishery disciplines: 900::Fisheries science: 920en_US
dc.subjectVDP::Landbruks- og Fiskerifag: 900::Fiskerifag: 920en_US
dc.titleOverexpression of T-bet, GATA-3 and TGF-ß induces IFN-γ, IL-4/13A, and IL-17A expression in Atlantic salmonen_US
dc.type.versionpublishedVersionen_US
dc.typeJournal articleen_US
dc.typeTidsskriftartikkelen_US
dc.typePeer revieweden_US


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