Show simple item record

dc.contributor.authorWolden, Runa
dc.contributor.authorPain, Maria
dc.contributor.authorKarlsson, Roger
dc.contributor.authorKarlsson, Anders
dc.contributor.authorFredheim, Elizabeth G. Aarag
dc.contributor.authorCavanagh, Jorunn Pauline
dc.date.accessioned2020-06-17T18:38:46Z
dc.date.available2020-06-17T18:38:46Z
dc.date.issued2020-04-07
dc.description.abstract<p><i>Background - </i>The skin commensal <i>Staphylococcus haemolyticus</i> is an emerging nosocomial pathogen. Despite its clinical relevance, published information about <i>S. haemolyticus</i> virulence factors is scarce. In this study, the adhesive and biofilm forming properties of ten clinical and ten commensal <i>S. haemolyticus</i> strains were examined using standard adhesion and biofilm assays. One of the clinical strains was used to identify expressed surface proteins using bacterial surface shaving. Protein abundance was examined by a comparative analysis between bacterial protein expression after human keratinocyte (HaCaT) colonization and growth in cell culture media supplemented with serum. Relative protein quantification was performed by labeling peptides with tandem mass tags (TMT) prior to Mass Spectrometry analysis. Surface proteins can be used as novel targets for antimicrobial treatment and in diagnostics. <p><i>Results - </i>Adherence to fibronectin, collagen and plastic was low in all tested strains, but with significantly higher adhesion to fibronectin (p = 0.041) and collagen (p = 0.001) in the commensal strains. There was a trend towards higher degree of biofilm formation in the clinical strains (p = 0.059). <p>By using surface shaving, 325 proteins were detected, of which 65 were classified as surface proteins. Analyses showed that the abundance of nineteen (5.8%) proteins were significantly changed following HaCaT colonization. The bacterial Toll/interleukin-1 like (TIRs) domain containing protein (p = 0.04), the transglycosylase SceD (p = 0.01), and the bifunctional autolysin Atl (p = 0.04) showed a 1.4, 1.6- and 1.5-fold increased abundance. The staphylococcal secretory antigen (SsaA) (p = 0.04) was significantly downregulated (− 1.5 fold change) following HaCaT colonization. <p>Among the 65 surface proteins the elastin binding protein (Ebps), LPXAG and LPXSG domain containing proteins and five LPXTG domain containing proteins were identified; three Sdr-like proteins, the extracellular matrix binding protein Embp and a SasH-like protein. <p><i>Conclusions - </i>This study has provided novel knowledge about expression of <i>S. haemolyticus</i> surface proteins after direct contact with eukaryotic cells and in media supplemented with serum. We have identified surface proteins and immune evasive proteins previously only functionally described in other staphylococcal species. The identification of expressed proteins after host-microbe interaction offers a tool for the discovery and design of novel targets for antimicrobial treatment.en_US
dc.identifier.citationWolden R, Pain MC, Karlsson, Karlsson, Fredheim E, Cavanagh JP, Karlson. Identification of surface proteins in a clinical Staphylococcus haemolyticus isolate by bacterial surface shaving. BMC Microbiology. 2020;20:80:1-18en_US
dc.identifier.cristinIDFRIDAID 1806017
dc.identifier.doi10.1186/s12866-020-01778-8
dc.identifier.issn1471-2180
dc.identifier.urihttps://hdl.handle.net/10037/18594
dc.language.isoengen_US
dc.publisherBMCen_US
dc.relation.ispartofWolden, R. (2024). Adhesion mechanisms and bacteriocins in <i>Staphylococcus haemolyticus</i> - New targets for the prevention and treatment of infections. (Doctoral thesis). <a href=https://hdl.handle.net/10037/34441>https://hdl.handle.net/10037/34441</a>.
dc.relation.journalBMC Microbiology
dc.rights.accessRightsopenAccessen_US
dc.rights.holderCopyright 2020 The Author(s)en_US
dc.subjectVDP::Medical disciplines: 700::Clinical medical disciplines: 750::Communicable diseases: 776en_US
dc.subjectVDP::Medisinske Fag: 700::Klinisk medisinske fag: 750::Infeksjonsmedisin: 776en_US
dc.titleIdentification of surface proteins in a clinical Staphylococcus haemolyticus isolate by bacterial surface shavingen_US
dc.type.versionpublishedVersionen_US
dc.typeJournal articleen_US
dc.typeTidsskriftartikkelen_US
dc.typePeer revieweden_US


File(s) in this item

Thumbnail

This item appears in the following collection(s)

Show simple item record