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dc.contributor.authorAhmad, Azeem
dc.contributor.authorDubey, Vishesh Kumar
dc.contributor.authorButola, Ankit
dc.contributor.authorTinguely, Jean-Claude
dc.contributor.authorAhluwalia, Balpreet Singh
dc.contributor.authorMehta, Dalip Singh
dc.date.accessioned2020-06-22T10:02:46Z
dc.date.available2020-06-22T10:02:46Z
dc.date.issued2020-03-18
dc.description.abstractPhase shifting interferometric (PSI) techniques are among the most sensitive phase measurement methods. Owing to its high sensitivity, any minute phase change caused due to environmental instability results into, inaccurate phase measurement. Consequently, a well calibrated piezo electric transducer (PZT) and highly-stable environment is mandatory for measuring accurate phase map using PSI implementation. Here, we present an inverse approach, which can retrieve phase maps of the samples with negligible errors under environmental fluctuations. The method is implemented by recording a video of continuous temporally phase shifted interferograms and phase shifts were calculated between all the data frames using Fourier transform algorithm with a high accuracy ≤ 5.5 × 10<sup>−4</sup> π rad. To demonstrate the robustness of the proposed method, a manual translation of the stage was employed to introduce continuous temporal phase shift between data frames. The developed algorithm is first verified by performing quantitative phase imaging of optical waveguide and red blood cells using uncalibrated PZT under the influence of vibrations/air turbulence and compared with the well calibrated PZT results. Furthermore, we demonstrated the potential of the proposed approach by acquiring the quantitative phase imaging of an optical waveguide with a rib height of only 2 nm and liver sinusoidal endothelial cells (LSECs). By using 12-bit CMOS camera the height of shallow rib waveguide is measured with a height sensitivity of 4 Å without using PZT and in presence of environmental fluctuations.en_US
dc.identifier.citationAhmad A, Dubey VK, Butola, Tinguely J, Ahluwalia BS, Mehta. Sub-nanometer height sensitivity by phase shifting interference microscopy under environmental fluctuations. Optics Express. 2020;28(7):9340-9358en_US
dc.identifier.cristinIDFRIDAID 1804019
dc.identifier.doi10.1364/OE.384259
dc.identifier.issn1094-4087
dc.identifier.urihttps://hdl.handle.net/10037/18617
dc.language.isoengen_US
dc.publisherOSA Publishingen_US
dc.relation.journalOptics Express
dc.relation.projectIDinfo:eu-repo/grantAgreement/EC/FP7/336716/EU/High-speed chip-based nanoscopy to discover real-time sub-cellular dynamics/NANOSCOPY/en_US
dc.relation.projectIDinfo:eu-repo/grantAgreement/RCN/BIOTEK2021/285571/Norway/Optimalisering: High-throughput and high-resolution pathology using chip-based nanoscopy//en_US
dc.relation.projectIDinfo:eu-repo/grantAgreement/RCN/NANO2021/288565/Norway/Integrated photonic chip-based nanoscopy for pathology & the clinic//en_US
dc.rights.accessRightsopenAccessen_US
dc.rights.holderCopyright 2020 The Author(s)en_US
dc.subjectVDP::Technology: 500en_US
dc.subjectVDP::Teknologi: 500en_US
dc.titleSub-nanometer height sensitivity by phase shifting interference microscopy under environmental fluctuationsen_US
dc.type.versionpublishedVersionen_US
dc.typeJournal articleen_US
dc.typeTidsskriftartikkelen_US
dc.typePeer revieweden_US


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