Blar i tittel Mastergradsoppgaver i biologi (Helsefak)
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“EFFECT OF IRRADIATION ON THE IMMUNOREGULATORY FUNCTIONS EXERTED BY TUMOUR FIBROBLASTS ON MACROPHAGES”
(Master thesis; Mastergradsoppgave, 2018-11-19)Summary Background/aim of the study: Carcinoma-associated fibroblasts (CAFs) represents a heterogeneous population of cells and are considered one of the dominant stromal component of solid tumours, performing a crucial role in tumour proliferation and metastasis. The main objective of this study was to investigate the immunoregulatory features of CAFs isolated from non-small cell lung carcinomas ... -
The effect of oral uptake of nicotine on skin blood perfusion of the face and hands in snus users as determined by thermography.
(Master thesis; Mastergradsoppgave, 2014-05-15)In recent years the number of people using cigarettes in Norway has significantly declined, with intake of nicotine being replaced by smokeless tobacco (ST) products such as snus placed in the oral cavity. While health risks from smoking cigarettes are well known little is known about health risks of using ST. The main aim of this thesis was to compare the effects of oral use of snus with nicotine ... -
The effect of sub-lethal concentration of ciprofloxacin on the transfer of multidrug resistance plasmids, fitness costs on the host and the stability of the newly acquired plasmids
(Master thesis; Mastergradsoppgave, 2014-09-30)The increase in reported cases of antimicrobial resistance has raised a lot of concerns in the public health community. This has prompted efforts aimed at finding out factors that have been contributing to this situation. In Gram-negative bacteria plasmids have been singled out as the most important vehicles behind the spread of antimicrobial resistance. Some plasmids can harbour several resistance ... -
Effect of viral early proteins, mutations and IL-17F on the transcriptional activity of the Merkel cell polyomavirus promoter in different cell lines
(Master thesis; Mastergradsoppgave, 2016-05-23)Merkel cell polyomavirus (MCPyV) is common in the human population with a seropositivity of approximately 60%. The virus is chronically shed from healthy skin, but the genuine host cell remains unknown and a permissive cell culture system is lacking. The viral genome is in an episomal state in cells where MCPyV has been found. The virus is not harmful in healthy individuals, but it is involved in ... -
Effects of exogenous hydrogen sulfide administration on cardiac function and reactive oxygen species production : a study in hearts from normal rats and rats with heart hypertrophy or ischemia
(Master thesis; Mastergradsoppgave, 2011-09)Coronary heart disease is the leading cause of death worldwide. Infarct size can be limited by interventions used after the ischemic event like the use of thrombolytic therapy or primary percutaneous coronary intervention. Paradoxically, however, the return of blood flow can also result in additional cardiac damage and complications, referred to as reperfusion injury. It has been shown that reperfusion ... -
Establishment of an advanced flow cytometry protocol for analyzing immune cell subsets from lung cancer patients
(Mastergradsoppgave; Master thesis, 2022-08-22)Lymphocytes are known for their role in tumor promotion and tumor suppression. T cell infiltration of the tumor microenvironment has been linked to a better prognosis and outcome in various malignancies. CD8+ T cells can eliminate cancer cells by causing them to lyse, while CD4+ T cells can be both tumor promoting and tumor suppressing. However, the prognostic impact of functional lymphocyte subtypes ... -
Et eksperimentelt studium av kreftceller fra livmorhals - Strålesensitivitet, effektutvikling og cGMP nivåer
(Master thesis; Mastergradsoppgave, 2008-06)I denne oppgaven ble kreftceller fra livmorhals bestrålt med gammastråler. Strålingens effekt på celletetthet, celledød og cellesyklus ble undersøkt for å si noe om disse cellenes strålesensitivitet. Den ekstracellulære konsentrasjonen av cGMP ble også målt. Dette sykliske nukleotidet har relevans fordi det har vist seg at nivået av cGMP går ned etter behandling hos pasienter med livmorhalskreft. -
Evaluation of the performance of two prediction models, the IrisPlex and the novel EC12 model, for eye colour predictions in a Norwegian population.
(Mastergradsoppgave; Master thesis, 2020-06-12)Biological material obtained from a crime scene is used to generate DNA-profile by typing short tandem repeat (STR) markers. However, if the STR-profile do not match the DNA profile of suspects or from a crime DNA database, the investigation can go towards typing markers that can estimate externally visible characteristics (EVCs). EVCs can function as a “biological witness” and thus aid a police ... -
Exploration of rabbit integrin β3 as a possible immune-tolerizing agent to prevent foetal and neonatal alloimmune thrombocytopenia.
(Mastergradsoppgave; Master thesis, 2020-08-07)Foetal and neonatal alloimmune thrombocytopenia (FNAIT) is a disease caused by the production of antibodies by the mother to the platelets of the foetus. The alloimmune response is caused by a P33L substitution in the Human Platelet Antigen-1a (HPA-1a) in a homo- or heterozygous HPA-1a foetus, in a homozygous HPA-1b+ mother. Maternal antibodies are produced by activated HPA-1a specific T-cells able ... -
Exploring the in vitro expansion of CD4 T cells. For improved culturing of CD4 T cells linked to FNAIT
(Master thesis; Mastergradsoppgave, 2018-05-15)Fetal and neonatal alloimmune thrombocytopenia (FNAIT) is a rare disease that may cause serious bleedings in the fetus or neonate of a woman who has developed antibodies against the fetus’ platelets. Development of FNAIT has been linked to the presence of platelet reactive CD4 T cells that help B cells to develop into antibody producing plasma cells. To be able to conduct research on such T cells, ... -
Expression and characterization of recombinant murine integrin β3 modified to express the human platelet antigen-1a epitope
(Mastergradsoppgave; Master thesis, 2017-05-14)Fetal and neonatal alloimmune thrombocytopenia (FNAIT) is a condition where the mother produces alloantibodies against fetal platelets during pregnancy most commonly due to an incompatibility in the human platelet antigen (HPA)-1 system; a single amino acid polymorphism located on integrin β3 with two common variants, HPA-1a and HPA-1b. Antibody responses against HPA-1a can be formed in women who ... -
Forensic DNA genotyping by means of next generation sequencing. Analysis of Autosomal STRs of a Norwegian population sample using the ForenSeq FGx system
(Master thesis; Mastergradsoppgave, 2018-05-15)Population databases containing allele frequencies of the genetic markers used for DNA-profiling are necessary for forensic geneticist to be able to perform statistical calculations on the statistical weight of DNA-evidence. However, allele frequencies differ from population to population, it is therefore important to establish population databases for specific graphical areas or population groups. ... -
Hereditary breast and ovarian cancer. Diversity of genetic causes of HBOC in a Norwegian breast and ovarian cancer patient cohort, BRCA2 c.8331+2C>T - a Norwegian founder mutation
(Master thesis; Mastergradsoppgave, 2017-05-14)Hereditary breast and ovarian cancer (HBOC) causes 5-10 % of breast cancer cases and 25 % of ovarian cancer cases. About 24 % of HBOC are caused by deleterious variants in BRCA1 and BRCA2. Currently, more than 25 different genes have been associated with HBOC, including BRCA1 and BRCA2, many of which encode proteins participating in homologous recombination repair (HRR) and mismatch repair (MMR). ... -
High Palmitate Alters Glutathione Redox During Severe Oxidant Stress in the Heart; An Evaluation of Altered Glutathione Redox Environment on Cardiac Function and Mitochondrial Energetics.
(Mastergradsoppgave; Master thesis, 2021-05-18)When the heart is under stress, e.g. ischemia-reperfusion (IR), it produces an enormous amount of reactive oxygen species (ROS). Glutathione, which is an endogenous antioxidant derived from the ‘nicotinamide adenine dinucleotide hydrogen’ (NADH) of the ‘tricarboxylic acid’ cycle, can fight against this oxidative stress to balance the redox environment, and convert the produced ROS to H2O. In this ... -
Human articular chondrocytes express Chemerin receptor, ChemR23, which conveys inflammatory signalling
(Master thesis; Mastergradsoppgave, 2008-12-17)Common features of arthritis include destruction of extracellular matrices in cartilage and bone as a result of chronic inflammation. Cartilage deterioration is generally described as a result from the effect of immune cells and their inflammatory mediators. However, recent reports suggest a role of chondrocytes in the initiation of inflammation in joints, and that they play a pivotal role in the ... -
The human polyomavirus KI: A study on cell permissivity, sub-cellular localization of VP1 and presence in cerebrospinal fluid and urine
(Master thesis; Mastergradsoppgave, 2011-11-17)KI polyomavirus (KIPyV) is a relatively newly discovered human polyomavirus originally identified in respiratory tract samples. Little is known about the route of infection or transmission, and the pathogenic properties of this virus are virtually unknown. The life cycle of KIPyV has not been studied as a permissive cell system has not been identified so far. The bona fide sites of viral replication ... -
Identification of protein interaction candidates for the GOLD domain of FYCO1
(Master thesis; Mastergradsoppgave, 2016-05-27)Autophagy is an evolutionary conserved degradative pathway, where damaged or surplus cytosolic components are sequestered into double membrane vesicles, autophagosomes, which become degraded through the lysosomal system. The autophagy is a dynamic process, which is depended of transport of autophagosomes along microtubule, to become degraded by lysosomes. One of the proteins involved in this transport ... -
Identification, isolation and characterisation of scavenging endothelial cells in placenta
(Master thesis; Mastergradsoppgave, 2014-05-15)Haemopoiesis occurs in the BM, where haemopoietic stem cells (HSC) reside in a three-dimensional “niche” and give rise to the millions of circulating blood cells required everyday of life. The precise regulation of this immense task occurs by interaction of HSC with other BM cells and extracellular matrix molecules. In addition, the BM vasculature has also now been identified as a key HSC regulator. ... -
Identifying Staphylococcus haemolyticus surface proteins. Development of a novel method for detection of bacterial surface proteins expressed during colonisation of human keratinocytes
(Master thesis; Mastergradsoppgave, 2018-05-15)The aim was to develop a method for identification of expressed surface proteins of Staphylococcus haemolyticus when colonising human keratinocytes. S. haemolyticus and HaCaT cells were grown together and separated with FACS prior to surface protein shaving using the LPI™FlowCell technology (Nanoxis Consulting AB). TMT-tags were used to investigate up- and downregulation of proteins comparing S. ... -
Immune-modulating properties of carcinoma-associated fibroblasts and their changes during radiotherapy. In vitro study of primary non-small cell lung carcinoma-associated fibroblasts
(Master thesis; Mastergradsoppgave, 2015-05-15)Background/aim of the study: Carcinoma-associated Fibroblasts (CAFs) comprise a heterogeneous population of cells and often represent the major cellular component of solid malignancies playing critical roles in tumor progression and metastasis. The purpose of this study was to gain more knowledge on the immunosuppressive traits of CAFs isolated from non-small cell lung carcinomas (NSCLC), and to ...