Mastergradsoppgaver i biologi (Helsefak): Recent submissions
Now showing items 21-40 of 76
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Molecular interaction studies of initial electrostatic attraction between trypsin and the human PAR-2 receptor
(Master thesis; Mastergradsoppgave, 2020-01-16)Workers in livestock and fish cultivation are at increased risk of occupational airway damage caused by proteases. Proteases, such as trypsin, activate PAR-2 which in turn triggers an inflammatory response, potentially causing airway damage over time. There has been some speculation that PAR-2 receptors easier attract trypsin form species where this enzyme has a more negative electrostatic charge. ... -
Regulation of urokinase receptor (uPAR) cleavage by cancer associated fibroblasts (CAFs) in oral cancer
(Master thesis; Mastergradsoppgave, 2019-05-15)Oral squamous cell carcinoma (OSCC) is one of the frequently diagnosed type of oral cancers and is a leading cause of cancer associated mortality and morbidity worldwide. Cancer associated fibroblasts (CAFs) are activated fibroblasts that are found in association with cancer cells. CAFs are the most abundant stromal cells in the tumor microenvironment (TME). In the TME, cell interactions mediated ... -
Identifying Staphylococcus haemolyticus surface proteins. Development of a novel method for detection of bacterial surface proteins expressed during colonisation of human keratinocytes
(Master thesis; Mastergradsoppgave, 2018-05-15)The aim was to develop a method for identification of expressed surface proteins of Staphylococcus haemolyticus when colonising human keratinocytes. S. haemolyticus and HaCaT cells were grown together and separated with FACS prior to surface protein shaving using the LPI™FlowCell technology (Nanoxis Consulting AB). TMT-tags were used to investigate up- and downregulation of proteins comparing S. ... -
Immune-modulating properties of carcinoma-associated fibroblasts and their changes during radiotherapy. In vitro study of primary non-small cell lung carcinoma-associated fibroblasts
(Master thesis; Mastergradsoppgave, 2015-05-15)Background/aim of the study: Carcinoma-associated Fibroblasts (CAFs) comprise a heterogeneous population of cells and often represent the major cellular component of solid malignancies playing critical roles in tumor progression and metastasis. The purpose of this study was to gain more knowledge on the immunosuppressive traits of CAFs isolated from non-small cell lung carcinomas (NSCLC), and to ... -
Study of human PRKAR1A and its role in autophagy
(Master thesis; Mastergradsoppgave, 2018-05-11)The human PRKAR1A gene is a 381- amino acid protein encoding the regulatory subunit (RIα) of the cAMP dependent Protein Kinase A (PKA). The main function of PRKAR1A is to regulate the catalytic activity of PKA. PRKAR1A is shown to regulate autophagy via association with mTOR kinase and colocalization with the autophagy marker proteins LC3B, Rab7 and Rab9. Contradictory findings regarding the ... -
Optimisation of high-resolution fluorescence microscopy of normal- and preeclamptic placentas
(Master thesis; Mastergradsoppgave, 2019-08-14)Preeclampsia (PE) affects 3-5 % of pregnant women and may lead to maternal and/or fetal death. The main theory of PE is placental ischemia, leading to a dysfunctional placenta and clinical signs as hypertension and proteinuria in the mother. The primary aim of the thesis was to implement and optimise a method for high-resolution microscopy of placental cryo-sections. Secondary aims were to compare ... -
Modulation of dendritic cells phenotype and functions by irradiated and non-irradiated lung cancer fibroblasts. An in vitro study.
(Master thesis; Mastergradsoppgave, 2019-05-15)Stromal cells in the tumor microenvironment (TME) interacts with cancer cells and affect their behavior in diverse manners. In this study, we investigated in vitro the mechanisms by which non-irradiated and irradiated cancer-associated fibroblasts (CAFs) isolated from patients with non-small cell lung carcinoma (NSCLC) affected the phenotype and function of monocyte-derived dendritic cells (Mo-DCs) ... -
Exploring the in vitro expansion of CD4 T cells. For improved culturing of CD4 T cells linked to FNAIT
(Master thesis; Mastergradsoppgave, 2018-05-15)Fetal and neonatal alloimmune thrombocytopenia (FNAIT) is a rare disease that may cause serious bleedings in the fetus or neonate of a woman who has developed antibodies against the fetus’ platelets. Development of FNAIT has been linked to the presence of platelet reactive CD4 T cells that help B cells to develop into antibody producing plasma cells. To be able to conduct research on such T cells, ... -
Identification of protein interaction candidates for the GOLD domain of FYCO1
(Master thesis; Mastergradsoppgave, 2016-05-27)Autophagy is an evolutionary conserved degradative pathway, where damaged or surplus cytosolic components are sequestered into double membrane vesicles, autophagosomes, which become degraded through the lysosomal system. The autophagy is a dynamic process, which is depended of transport of autophagosomes along microtubule, to become degraded by lysosomes. One of the proteins involved in this transport ... -
Forensic DNA genotyping by means of next generation sequencing. Analysis of Autosomal STRs of a Norwegian population sample using the ForenSeq FGx system
(Master thesis; Mastergradsoppgave, 2018-05-15)Population databases containing allele frequencies of the genetic markers used for DNA-profiling are necessary for forensic geneticist to be able to perform statistical calculations on the statistical weight of DNA-evidence. However, allele frequencies differ from population to population, it is therefore important to establish population databases for specific graphical areas or population groups. ... -
The Immunostimulatory Potential of Mesenchymal Stem Cells for formation of Tertiary Lymphoid Structures in Lupus Nephritis
(Master thesis; Mastergradsoppgave, 2017-05-15)Formation of tertiary lymphoid structures (TLS) occurs in tissues targeted by chronic inflammatory processes, such as infection and autoimmunity. In systemic lupus erythematosus (SLE), TLS have been observed in the kidneys of lupus-prone mice and in kidney biopsies of SLE patients with Lupus Nephritis (LN). Here the role of tissue-specific mesenchymal stem cells (MSCs) as lymphoid tissue organizer ... -
The population structure of human carriage and clinical isolates of ESBL-producing Escherichia coli and Klebsiella pneumoniae in Norway
(Master thesis; Mastergradsoppgave, 2017-05-15)The increasing emergence of antimicrobial resistant bacteria worldwide is recognised as a severe threat to public health on a global scale. Without effective antimicrobial agents to treat bacterial infections, modern medicine will be set back several decades and deaths caused by bacterial infections will increase. The most widely used class of antimicrobials, is -lactams, and the increase in resistance ... -
Computational study of SERT using new X-ray crystal structures and initial experimental verification of potential GABAB receptor compounds
(Master thesis; Mastergradsoppgave, 2018-05-15)Depression is currently one of the leading causes worldwide of suicide and disability, the most common treatment is antidepressants. Most antidepressants work by increasing the monoamine levels in the central nervous system by inhibition of the reuptake of monoamines into the presynaptic neuron, and thereby ensure accumulation of the monoamines in the synaptic cleft. The main target for most ... -
“EFFECT OF IRRADIATION ON THE IMMUNOREGULATORY FUNCTIONS EXERTED BY TUMOUR FIBROBLASTS ON MACROPHAGES”
(Master thesis; Mastergradsoppgave, 2018-11-19)Summary Background/aim of the study: Carcinoma-associated fibroblasts (CAFs) represents a heterogeneous population of cells and are considered one of the dominant stromal component of solid tumours, performing a crucial role in tumour proliferation and metastasis. The main objective of this study was to investigate the immunoregulatory features of CAFs isolated from non-small cell lung carcinomas ... -
Characterization of ATG8 Family Protein Binding to TRIM23 and TRIM31
(Master thesis; Mastergradsoppgave, 2018-05-15)Macroautophagy (hereafter called autophagy) is a process where proteins, organelles and intracellular micro-organisms are degraded by the lysosome. The autophagosome engulfs a part of the cytoplasm where the cargo is, and transports it to the endosomal-lysosomal system for degradation. Autophagy can also be selective, where cargos are recognized directly by the autophagy receptors or by other proteins ... -
In vitro reconstitution of proMatrix Metalloproteinase-9/Chondroitin Sulfate Proteoglycan Complexes. Identification of motifs in proMMP-9 and the serglycin core protein involved in the complex formation.
(Master thesis; Mastergradsoppgave, 2014-05-15)Previously it has been shown that different monocytic leukemic cell lines such as THP-1, MonoMac and U-937 can produce proteolytic enzymes such as MMP-9 as well as various types of proteoglycans (PG). These proteins have been shown to be involved in homeostasis as well as in various diseases, such as cancer. When proMMP-9 was mixed with isolated PGs from these three cell lines or pure serglycin and ... -
Hereditary breast and ovarian cancer. Diversity of genetic causes of HBOC in a Norwegian breast and ovarian cancer patient cohort, BRCA2 c.8331+2C>T - a Norwegian founder mutation
(Master thesis; Mastergradsoppgave, 2017-05-14)Hereditary breast and ovarian cancer (HBOC) causes 5-10 % of breast cancer cases and 25 % of ovarian cancer cases. About 24 % of HBOC are caused by deleterious variants in BRCA1 and BRCA2. Currently, more than 25 different genes have been associated with HBOC, including BRCA1 and BRCA2, many of which encode proteins participating in homologous recombination repair (HRR) and mismatch repair (MMR). ... -
The oncoproteins gelsolin, periostin and thrombospondin are enriched in Merkel cell carcinoma exosomes, and their promoter activity is stimulated by Large T-antigen of Merkel cell polyomavirus
(Master thesis; Mastergradsoppgave, 2017-05-14)Merkel cell carcinoma (MCC) is a rare, aggressive neuroendocrine form of skin cancer with high mortality rate and increasing incidence. Around 80 % of MCC tumours are positive for Merkel cell polyomavirus (MCPyV), the only polyomavirus known to induce cancer in humans. Continuous expression of the viral oncogenes LT-ag and st-ag is necessary for MCPyV positive MCC tumour cell survival, suggesting ... -
Analysis of glomerular target structures for nephritic anti-dsDNA antibodies playing central roles in lupus nephritis
(Master thesis; Mastergradsoppgave, 2008-05)Systemic lupus erythematosus is an autoimmune disease characterized by the presence of autoantibodies towards nuclear antigens. Sub-populations of anti-dsDNA antibodies have the potential to bind in the kidney and thereby induce nephritis, but there is no definite distinction that separates non-pathogenic from pathogenic antibodies. There must be parameters that determine anti-dsDNA antibody binding, ... -
Production and purification of recombinant C-terminal truncated pro-Matrix Metalloprotease-9
(Master thesis; Mastergradsoppgave, 2016-06-21)MMP-9 consists of N-terminal signal peptide, a pro-domain, catalytic domain, a long flexible hinge region and C- terminal hemopexin domain. In the present study a truncated version of proMMP-9 which lacking hinge region and C-terminal hemopexin domain was produced. To do so Sf9 insect cells and the BaculoDirect Baculovirus expression system were used. Using site directed mutagenesis, single mutation ...