dc.contributor.author | Håkonsholm, Fredrik | |
dc.contributor.author | Hetland, Marit A.K. | |
dc.contributor.author | Svanevik, Cecilie Smith | |
dc.contributor.author | Sundsfjord, Arnfinn | |
dc.contributor.author | Lunestad, Bjørn Tore | |
dc.contributor.author | Marathe, Nachiket | |
dc.date.accessioned | 2021-01-07T14:39:08Z | |
dc.date.available | 2021-01-07T14:39:08Z | |
dc.date.issued | 2020-11-30 | |
dc.description.abstract | <i>Klebsiella</i> spp. are a major cause of both nosocomial and community acquired infections, with <i>K. pneumoniae</i> being responsible for most human infections. Although <i>Klebsiella</i> spp. are present in a variety of environments, their distribution in the sea and the associated antibiotic resistance is largely unknown. In order to examine prevalence of <i>K. pneumoniae</i> and related species in the marine environment, we sampled 476 batches of marine bivalve molluscs collected along the Norwegian coast. From these samples, <i>K. pneumoniae</i> (<i>n</i> = 78), <i>K. oxytoca</i> (<i>n</i> = 41), <i>K. variicola</i> (<i>n</i> = 33), <i>K. aerogenes</i> (<i>n</i> = 1), <i>Raoultella ornithinolytica</i> (<i>n</i> = 38) and <i>R. planticola</i> (<i>n</i> = 13) were isolated. The number of positive samples increased with higher levels of faecal contamination. We found low prevalence of acquired resistance in all isolates, with seven <i>K. pneumoniae</i> isolates showing resistance to more than one antibiotic class. The complete genome sequence of cefotaxime-resistant <i>K. pneumoniae</i> sensu stricto isolate 2016-1400 was obtained using Oxford Nanopore and Illumina MiSeq based sequencing. The 2016-1400 genome had two contigs, one chromosome of 5,088,943 bp and one plasmid of 191,744 bp and belonged to ST1035. The β-lactamase genes <i>bla</i><sub>CTX-M-3</sub> and <i>bla</i><sub>TEM-1</sub>, as well as the heavy metal resistance genes pco, ars and sil were carried on a plasmid highly similar to one found in <i>K. pneumoniae</i> strain C17KP0055 from South-Korea recovered from a blood stream infection. The present study demonstrates that <i>K. pneumoniae</i> are prevalent in the coastal marine environment and that bivalve molluscs may act as a potential reservoir of extended spectrum β-lactamase (ESBL)-producing <i>K. pneumoniae</i> that may be transmitted through the food chain. | en_US |
dc.identifier.citation | Håkonsholm, Hetland, Svanevik, Sundsfjord, Lunestad, Marathe. Antibiotic sensitivity screening of Klebsiella spp. and Raoultella spp. isolated from marine bivalve molluscs reveal presence of CTX-M-producing K. pneumoniae. Microorganisms. 2020;8:1909(12):1-15 | en_US |
dc.identifier.cristinID | FRIDAID 1859012 | |
dc.identifier.doi | 10.3390/microorganisms8121909 | |
dc.identifier.issn | 2076-2607 | |
dc.identifier.uri | https://hdl.handle.net/10037/20206 | |
dc.language.iso | eng | en_US |
dc.publisher | MDPI | en_US |
dc.relation.ispartof | Håkonsholm, F. (2023). <i>Klebsiella pneumoniae</i> in the marine environment. (Doctoral thesis). <a href=https://hdl.handle.net/10037/28919>https://hdl.handle.net/10037/28919</a> | |
dc.relation.journal | Microorganisms | |
dc.rights.accessRights | openAccess | en_US |
dc.rights.holder | Copyright 2020 The Author(s) | en_US |
dc.subject | VDP::Medical disciplines: 700::Basic medical, dental and veterinary science disciplines: 710 | en_US |
dc.subject | VDP::Medisinske Fag: 700::Basale medisinske, odontologiske og veterinærmedisinske fag: 710 | en_US |
dc.title | Antibiotic sensitivity screening of Klebsiella spp. and Raoultella spp. isolated from marine bivalve molluscs reveal presence of CTX-M-producing K. pneumoniae | en_US |
dc.type.version | publishedVersion | en_US |
dc.type | Journal article | en_US |
dc.type | Tidsskriftartikkel | en_US |
dc.type | Peer reviewed | en_US |