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dc.contributor.authorDawadi, Rangita
dc.contributor.authorMalla, Nabin
dc.contributor.authorHegge, Beate
dc.contributor.authorWushur, Imin
dc.contributor.authorBerg, Eli
dc.contributor.authorSvineng, Gunbjørg
dc.contributor.authorSylte, Ingebrigt
dc.contributor.authorWinberg, Jan-Olof
dc.date.accessioned2021-01-22T09:43:59Z
dc.date.available2021-01-22T09:43:59Z
dc.date.issued2020-06-12
dc.description.abstractPrevious studies have shown that THP-1 cells produced an SDS-stable and reduction-sensitive complex between proMMP-9 and a chondroitin sulfate proteoglycan (CSPG) core protein. The complex could be reconstituted in vitro using purified serglycin (SG) and proMMP-9 and contained no inter-disulfide bridges. It was suggested that the complex involved both the FnII module and HPX domain of proMMP-9. The aims of the present study were to resolve the interacting regions of the molecules that form the complex and the types of interactions involved. In order to study this, we expressed and purified full-length and deletion variants of proMMP-9, purified CSPG and SG, and performed in vitro reconstitution assays, peptide arrays, protein modelling, docking, and molecular dynamics (MD) simulations. ProMMP-9 variants lacking both the FnII module and the HPX domain did not form the proMMP-9∙CSPG/SG complex. Deletion variants containing at least the FnII module or the HPX domain formed the proMMP-9∙CSPG/SG complex, as did the SG core protein without CS chains. The interacting parts covered large surface areas of both molecules and implicated dynamic and complementary ionic, hydrophobic, and hydrogen bond interactions. Hence, no short single interacting linear motifs in the two macromolecules could explain the strong SDS-stable and reduction-sensitive binding.en_US
dc.identifier.citationDawadi R, Malla N, Hegge B, Wushur W, Berg E, Svineng g, Sylte IS, Winberg J-O. Molecular Interactions Stabilizing the Promatrix Metalloprotease-9·Serglycin Heteromer. International Journal of Molecular Sciences. 2020;21(12):4205
dc.identifier.cristinIDFRIDAID 1861385
dc.identifier.doi10.3390/ijms21124205
dc.identifier.issn1422-0067
dc.identifier.urihttps://hdl.handle.net/10037/20384
dc.language.isoengen_US
dc.publisherMDPIen_US
dc.relation.journalInternational Journal of Molecular Sciences
dc.rights.holderCopyright 2020 The Author(s)en_US
dc.subjectVDP::Medical disciplines: 700::Basic medical, dental and veterinary science disciplines: 710en_US
dc.subjectVDP::Medisinske Fag: 700::Basale medisinske, odontologiske og veterinærmedisinske fag: 710en_US
dc.titleMolecular Interactions Stabilizing the Promatrix Metalloprotease-9·Serglycin Heteromeren_US
dc.type.versionpublishedVersionen_US
dc.typeJournal articleen_US
dc.typeTidsskriftartikkelen_US
dc.typePeer revieweden_US


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