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dc.contributor.authorHuijgens, Patty Thalia
dc.contributor.authorSnoeren, Eelke
dc.contributor.authorMeisel, Robert
dc.contributor.authorMermelstein, Paul
dc.date.accessioned2021-01-25T10:21:42Z
dc.date.available2021-01-25T10:21:42Z
dc.date.issued2020-11-06
dc.description.abstractGonadal hormones affect neuronal morphology to ultimately regulate behaviour. In female rats, oestradiol mediates spine plasticity in hypothalamic and limbic brain structures, contributing to long‐lasting effects on motivated behaviour. Parallel effects of androgens in male rats have not been extensively studied. Here, we investigated the effect of both castration and androgen replacement on spine plasticity in the nucleus accumbens shell and core (NAcSh and NAcC), caudate putamen (CPu), medial amygdala (MeA) and medial preoptic nucleus (MPN). Intact and castrated (gonadectomy [GDX]) male rats were treated with dihydrotestosterone (DHT, 1.5 mg) or vehicle (oil) in three experimental groups: intact‐oil, GDX‐oil and GDX‐DHT. Spine density and morphology, measured 24 hours after injection, were determined through three‐dimensional reconstruction of confocal z‐stacks of DiI‐labelled dendritic segments. We found that GDX decreased spine density in the MPN, which was rescued by DHT treatment. DHT also increased spine density in the MeA in GDX animals compared to intact oil‐treated animals. By contrast, DHT decreased spine density in the NAcSh compared to GDX males. No effect on spine density was observed in the NAcC or CPu. Spine length and spine head diameter were unaffected by GDX and DHT in the investigated brain regions. In addition, immunohistochemistry revealed that DHT treatment of GDX animals rapidly increased the number of cell bodies in the NAcSh positive for phosphorylated cAMP response‐element binding protein, a downstream messenger of the androgen receptor. These findings indicate that androgen signalling plays a role in the regulation of spine plasticity within neurocircuits involved in motivated behaviours.en_US
dc.identifier.citationHuijgens PT, Snoeren E, Meisel R, Mermelstein P. Effects of gonadectomy and dihydrotestosterone on neuronal plasticity in motivation and reward related brain regions in the male rat. Journal of neuroendocrinology. 2020en_US
dc.identifier.cristinIDFRIDAID 1854014
dc.identifier.doihttps://doi.org/10.1111/jne.12918
dc.identifier.issn0953-8194
dc.identifier.issn1365-2826
dc.identifier.urihttps://hdl.handle.net/10037/20457
dc.language.isoengen_US
dc.publisherWileyen_US
dc.relation.ispartofHuijgens, P.T. (2021). Organization and orchestration of male rat sexual behavior. (Doctoral thesis). <a href=https://hdl.handle.net/10037/22145>https://hdl.handle.net/10037/22145</a>.
dc.relation.journalJournal of neuroendocrinology
dc.relation.projectIDinfo:eu-repo/grantAgreement/RCN/FRIMEDBIO/251320/Norway/Which specific brain projections are involved in natural reward?//en_US
dc.rights.accessRightsopenAccessen_US
dc.rights.holderCopyright 2020 The Author(s)en_US
dc.subjectVDP::Social science: 200::Psychology: 260en_US
dc.subjectVDP::Samfunnsvitenskap: 200::Psykologi: 260en_US
dc.titleEffects of gonadectomy and dihydrotestosterone on neuronal plasticity in motivation and reward related brain regions in the male raten_US
dc.type.versionpublishedVersionen_US
dc.typeJournal articleen_US
dc.typeTidsskriftartikkelen_US
dc.typePeer revieweden_US


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