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dc.contributor.authorNthiga, Thaddaeus Mutugi
dc.contributor.authorShrestha, Birendra Kumar
dc.contributor.authorSjøttem, Eva
dc.contributor.authorBruun, Jack-Ansgar
dc.contributor.authorLarsen, Kenneth Bowitz
dc.contributor.authorBhujabal, Zambarlal
dc.contributor.authorLamark, Trond
dc.contributor.authorJohansen, Terje
dc.date.accessioned2021-03-09T09:56:35Z
dc.date.available2021-03-09T09:56:35Z
dc.date.issued2020-06-11
dc.description.abstractThe endoplasmic reticulum (ER) plays important roles in protein synthesis and folding, and calcium storage. The volume of the ER and expression of its resident proteins are increased in response to nutrient stress. ER‐phagy, a selective form of autophagy, is involved in the degradation of the excess components of the ER to restore homeostasis. Six ER‐resident proteins have been identified as ER‐phagy receptors so far. In this study, we have identified CALCOCO1 as a novel ER‐phagy receptor for the degradation of the tubular ER in response to proteotoxic and nutrient stress. CALCOCO1 is a homomeric protein that binds directly to ATG8 proteins via LIR‐ and UDS‐interacting region (UIR) motifs acting co‐dependently. CALCOCO1‐mediated ER‐phagy requires interaction with VAMP‐associated proteins VAPA and VAPB on the ER membranes via a conserved FFAT‐like motif. Depletion of CALCOCO1 causes expansion of the ER and inefficient basal autophagy flux. Unlike the other ER‐phagy receptors, CALCOCO1 is peripherally associated with the ER. Therefore, we define CALCOCO1 as a soluble ER‐phagy receptor.en_US
dc.identifier.citationNthiga, Shrestha BK, Sjøttem, Bruun, Larsen, Bhujabal, Lamark, Johansen. CALCOCO1 acts with VAMP ‐associated proteins to mediate ER ‐phagy. EMBO Journal. 2020en_US
dc.identifier.cristinIDFRIDAID 1815123
dc.identifier.doi10.15252/embj.2019103649
dc.identifier.issn0261-4189
dc.identifier.issn1460-2075
dc.identifier.urihttps://hdl.handle.net/10037/20659
dc.language.isoengen_US
dc.publisherEMBO Pressen_US
dc.relation.journalEMBO Journal
dc.relation.projectIDinfo:eu-repo/grantAgreement/RCN/FRIMEDBIO/214448/Norway/Selective autophagy and cell signalling: Regulation of p62/SQSTM1 and functional studies of novel LIR-containing proteins//en_US
dc.relation.projectIDinfo:eu-repo/grantAgreement/RCN/FRIMEDBIO/249884/Norway/Autophagy-regulated Signalosomes in Cellular Stress and Disease Pathways//en_US
dc.rights.accessRightsopenAccessen_US
dc.rights.holderCopyright 2020 The Author(s)en_US
dc.subjectVDP::Medical disciplines: 700::Basic medical, dental and veterinary science disciplines: 710en_US
dc.subjectVDP::Medisinske Fag: 700::Basale medisinske, odontologiske og veterinærmedisinske fag: 710en_US
dc.titleCALCOCO1 acts with VAMP ‐associated proteins to mediate ER ‐phagyen_US
dc.type.versionpublishedVersionen_US
dc.typeJournal articleen_US
dc.typeTidsskriftartikkelen_US
dc.typePeer revieweden_US


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