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dc.contributor.authorNúñez-Egido, Sandra
dc.contributor.authorLowther, Andrew
dc.contributor.authorNymo, Ingebørg H.
dc.contributor.authorKlein, Jörn
dc.contributor.authorBreines, Eva Marie
dc.contributor.authorTryland, Morten
dc.date.accessioned2021-03-24T08:15:18Z
dc.date.available2021-03-24T08:15:18Z
dc.date.issued2020-12-17
dc.description.abstractKnowledge of the health status and potential effect of disease outbreaks among Southern Ocean fauna may be decisive for its conservation. We assessed the exposure and infection of Antarctic fur seals (<i>Arctocephalus gazella</i>, AFS) and Southern elephant seals (<i>Mirounga leonine</i>, SES) to parapoxvirus, <i>Phocid alphaherpesvirus-1</i> (PhHV-1), smooth <i>Brucella</i> spp. and <i>Toxoplasma gondii</i>. AFS (<i>n</i> = 65) serum and swab samples, and SES (<i>n</i> = 13) serum samples from the sub--Antarctic island of Bouvetøya (54°25’S, 03°22’E) were collected during two austral summers (2014/15, 2017/18). Three polymerase chain reaction (PCR) tests amplifying the DNA polymerase, <i>B2L</i> and <i>GIF</i> parapoxvirus genomic regions were performed, investigating DNA from mucosal swab samples. The glycoprotein B gene was targeted to detect PhHV-1 viral DNA. Sera were assayed for <i>T. gondii</i> and smooth <i>Brucella</i> spp. antibodies with indirect enzyme-linked immunosorbent assays. Parapoxvirus PCR amplicons of the expected size were generated in two of the 29 AFS pups (nasal swabs, 2014/15), targeting the <i>B2L</i> (<i>n</i> = 2) and DNA polymerase (<i>n</i> = 1) genes, whereas the <i>GIF</i> PCR did not amplify target sequences. The PCR amplicons were sequenced and blasted in GenBank, revealing highest similarity with a seal parapoxvirus, confirming the presence of the virus in AFS for the first time. No PhHV-1 amplicons were generated, and antibodies against <i>T. gondii</i> or smooth <i>Brucella</i> spp. were not detected. Our data indicate that these seals are host for parapoxvirus but are neither exposed to smooth <i>Brucella</i> spp. nor <i>T. gondii</i>. Evidence of PhHV-1 shedding was not detected.en_US
dc.identifier.citationNúñez-Egido, Lowther, Nymo, Klein, Breines EM, Tryland M. Pathogen surveillance in Southern Ocean pinnipeds. Polar Research. 2020;39:1-11en_US
dc.identifier.cristinIDFRIDAID 1865797
dc.identifier.doi10.33265/polar.v39.3841
dc.identifier.issn0800-0395
dc.identifier.issn1751-8369
dc.identifier.urihttps://hdl.handle.net/10037/20722
dc.language.isoengen_US
dc.publisherNorsk Polarinstitutten_US
dc.relation.journalPolar Research
dc.rights.accessRightsopenAccessen_US
dc.rights.holderCopyright 2020 The Author(s)en_US
dc.subjectDyrehelse / Animal healthen_US
dc.subjectSel / Pinnipedsen_US
dc.subjectVDP::Mathematics and natural science: 400::Zoology and botany: 480en_US
dc.subjectVDP::Matematikk og Naturvitenskap: 400::Zoologiske og botaniske fag: 480en_US
dc.titlePathogen surveillance in Southern Ocean pinnipedsen_US
dc.type.versionpublishedVersionen_US
dc.typeJournal articleen_US
dc.typeTidsskriftartikkelen_US
dc.typePeer revieweden_US


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