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dc.contributor.authorUeland, Nora Lamark
dc.contributor.authorLudvigsen, Judith K.
dc.contributor.authorHellerud, Bernt C
dc.contributor.authorMollnes, Tom Eirik
dc.contributor.authorSkjeflo, Espen Waage
dc.date.accessioned2021-04-19T07:53:52Z
dc.date.available2021-04-19T07:53:52Z
dc.date.issued2020-10-06
dc.description.abstract<i>Background</i> - In order to adequately monitor cytokines in experimental models, currently available methods and commercially available kits should be compared.<br><br> <i>Aim</i> - To compare the plasma and tissue concentrations of IL-1β, IL-6, IL-8, IL-10, and TNF as a measure of systemic inflammation in septic pigs.<br><br> <i>Methods</i> - Cytokines were quantified from blood and tissue samples obtained at 0, 60, 120, 180, and 240 min, and in postmortem biopsies of the liver, kidney, lung, heart, and spleen from 26 anesthetized landrace pigs. (24 with experimental sepsis, two sham controls). Porcine-specific ELISAs (R&D) and multiplex (9-plex from Thermo Fischer, 13-plex from Millipore) immunoassays were compared.<br><br> <i>Results</i> - The assays differed for the different cytokines and between blood and tissue. In blood, the highest concentration of TNF and IL-6 was in ELISA, IL-1β equal in ELISA and 13-plex, IL-8 in 13-plex and IL-10 in 9-plex. In tissue, the highest concentration of TNF and IL-1β was in ELISA, IL-6 and IL-8 in 13-plex and IL-10 in 9-plex.<br><br> <i>Conclusion</i> - The choice of analysis impacts the quantified cytokine responses in porcine models. ELISA and multiplex techniques supplement each other and our data suggest which assays to use for the quantification of the different cytokines.en_US
dc.descriptionAccepted manuscript version, licensed <a href=http://creativecommons.org/licenses/by-nc-nd/4.0/> CC BY-NC-ND 4.0. </a>en_US
dc.identifier.citationUeland NL, Ludvigsen, Hellerud BC, Mollnes, Skjeflo EW. Choice of immunoassay to evaluate porcine cytokine levels. Veterinary Immunology and Immunopathology. 2020;230:110129:1-5en_US
dc.identifier.cristinIDFRIDAID 1873491
dc.identifier.doi10.1016/j.vetimm.2020.110129
dc.identifier.issn0165-2427
dc.identifier.issn1873-2534
dc.identifier.urihttps://hdl.handle.net/10037/20921
dc.language.isoengen_US
dc.publisherElsevieren_US
dc.relation.journalVeterinary Immunology and Immunopathology
dc.relation.projectIDEC/FP7: 602699en_US
dc.relation.projectIDinfo:eu-repo/grantAgreement/EC/FP7/602699/EU/Disarming the intravascular innate immune response to improve treatment modalities for chronic kidney disease/DIREKT/en_US
dc.rights.accessRightsopenAccessen_US
dc.rights.holder© 2020 Elsevier B.V. All rights reserved.en_US
dc.subjectVDP::Medical disciplines: 700en_US
dc.subjectVDP::Medisinske Fag: 700en_US
dc.titleChoice of immunoassay to evaluate porcine cytokine levelsen_US
dc.type.versionacceptedVersionen_US
dc.typeJournal articleen_US
dc.typeTidsskriftartikkelen_US
dc.typePeer revieweden_US


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