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dc.contributor.authorCauzzo, Jennifer
dc.contributor.authorNystad, Mona
dc.contributor.authorHolsæter, Ann Mari
dc.contributor.authorBasnet, Purusotam
dc.contributor.authorSkalko-Basnet, Natasa
dc.date.accessioned2021-05-04T09:03:25Z
dc.date.available2021-05-04T09:03:25Z
dc.date.issued2020-07-09
dc.description.abstractThe rather limited success of translation from basic research to clinical application has been highlighted as a major issue in the nanomedicine field. To identify the factors influencing the applicability of nanosystems as drug carriers and potential nanomedicine, we focused on following their fate through fluorescence-based assays, namely flow cytometry and imaging. These methods are often used to follow the nanocarrier internalization and targeting; however, the validity of the obtained results strictly depends on how much the nanosystem’s fate can be inferred from the fate of fluorescent dyes. To evaluate the parameters that affect the physicochemical and biological stability of the labeled nanosystems, we studied the versatility of two lipid dyes, TopFluor®-PC and Cy5-DSPE, in conventional liposomes utilizing well-defined in vitro assays. Our results suggest that the dye can affect the major characteristics of the system, such as vesicle size and zeta-potential. However, a nanocarrier can also affect the dye properties. Medium, temperature, time, fluorophore localization and its concentration, as well as their interplay, affect the outcome of tracing experiments. Therefore, an in-depth characterization of the labeled nanosystem should be fundamental to understand the conditions that validate the results within the screening process in optimization of nanocarrier.en_US
dc.identifier.citationCauzzo, J., Nystad, M., Holsæter, A. M., Basnet, P. & Škalko-Basnet, N. (2020). Following the Fate of Dye-Containing Liposomes In Vitro. <i>International Journal of Molecular Sciences, 21</i>(14).<a href=https://doi.org/10.3390/ijms21144847>https://doi.org/10.3390/ijms21144847</a>en_US
dc.identifier.cristinIDFRIDAID 1819518
dc.identifier.doihttps://doi.org/10.3390/ijms21144847
dc.identifier.urihttps://hdl.handle.net/10037/21141
dc.language.isoengen_US
dc.publisherMDPIen_US
dc.relation.projectIDEU: 766181en_US
dc.relation.projectIDEC/H2020: 766181en_US
dc.relation.projectIDinfo:eu-repo/grantAgreement/EC/H2020/766181/EU/Super-resolution optical microscopy of nanosized pore dynamics in endothelial cells/DeLIVER/en_US
dc.rights.accessRightsopenAccessen_US
dc.rights.holderCopyright 2020 The Author(s)en_US
dc.subjectVDP::Medical disciplines: 700en_US
dc.subjectVDP::Medisinske Fag: 700en_US
dc.titleDyes in Disguise: characterization of fluorescently labeled liposomesen_US
dc.type.versionpublishedVersionen_US
dc.typeJournal articleen_US
dc.typeTidsskriftartikkelen_US
dc.typePeer revieweden_US


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