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dc.contributor.advisorPerander, Maria
dc.contributor.authorWian, Barbro Lyngedal
dc.date.accessioned2021-06-01T10:39:02Z
dc.date.available2021-06-01T10:39:02Z
dc.date.issued2016-06-01en
dc.description.abstractBreast cancer represents 22 % of all cancer cases amongst the female population in Norway, which makes it by far the most common form of cancer in this group. It is a heterogeneous disease, and to guide use of adjuvant treatment, breast cancer patients are categorized into different subgroups based on biomarkers, which in turn enables specific target treatment options. Long non-coding RNAs (lncRNA) are previously unexplored transcripts from the mammalian genome, but are currently receiving increased attention, as it potentially can give rise to novel biomarkers and new targets for medical treatment amongst cancer patients in the future. Nuclear enriched abundant transcript 1 (NEAT1) is an lncRNA that proves to be involved in tumorgenesis, as induction of it leads to accelerated cellular proliferation, improved clonogenic survival and reduced apoptosis. High tumor NEAT1 expression also correlates with pore survival in patients with breast cancer. I have worked with two different cell lines, HeLa cells which are human cervical epithelial cells, and SK-BR-3 cells from human adenocarcinoma in breasts. I have pre-treated them with two different drugs, Gefitinib and Trastuzumab, and stimulated them with epidermal growth factor (EGF). By RNA isolation and RT-qPCR I’ve detected the expression of NEAT1_2 relative to GADPH, which is a constitutively expressed housekeeping ncRNA. I’ve also done Western Blot to detect ERK- and phosphorylated ERK proteins, to verify that EGF succeeded in stimulating the cells though activation of the Ras-MAPK signalling pathway.en_US
dc.identifier.urihttps://hdl.handle.net/10037/21309
dc.language.isoengen_US
dc.publisherUiT Norges arktiske universitetno
dc.publisherUiT The Arctic University of Norwayen
dc.rights.holderCopyright 2016 The Author(s)
dc.rights.urihttps://creativecommons.org/licenses/by-nc-sa/3.0en_US
dc.rightsAttribution-NonCommercial-ShareAlike 3.0 Unported (CC BY-NC-SA 3.0)en_US
dc.subject.courseIDMED-3950
dc.subjectVDP::Medisinske Fag: 700::Basale medisinske, odontologiske og veterinærmedisinske fag: 710::Medisinsk molekylærbiologi: 711en_US
dc.subjectVDP::Medical disciplines: 700::Basic medical, dental and veterinary science disciplines: 710::Medical molecular biology: 711en_US
dc.titleLong non-coding RNA and breast canceren_US
dc.typeMaster thesisen
dc.typeMastergradsoppgaveno


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Attribution-NonCommercial-ShareAlike 3.0 Unported (CC BY-NC-SA 3.0)
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