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dc.contributor.authorAmundsen, Marit
dc.contributor.authorTartor, Haitham
dc.contributor.authorAndersen, Kathrine
dc.contributor.authorSveinsson, Karoline Overn
dc.contributor.authorThoen, Even
dc.contributor.authorGjessing, Mona Cecilie
dc.contributor.authorDahle, Maria
dc.date.accessioned2021-06-15T11:19:26Z
dc.date.available2021-06-15T11:19:26Z
dc.date.issued2021-06-09
dc.description.abstractSalmon Gill Poxvirus Disease (SGPVD) has emerged as a cause of acute mortality in Atlantic salmon (Salmo salar L.) presmolts in Norwegian aquaculture. The clinical phase of the disease is associated with apoptotic cell death in the gill epithelium causing acute respiratory distress, followed by proliferative changes in the regenerating gill in the period after the disease outbreak. In an experimental SGPV challenge trial published in 2020, acute disease was only seen in fish injected with hydrocortisone 24 h prior to infection. SGPV-mediated mortality in the hydrocortisone-injected group was associated with more extensive gill pathology and higher SGPV levels compared to the group infected with SGPV only. In this study based on the same trial, SGPV gene expression and the innate and adaptive antiviral immune response was monitored in gills and spleen in the presence and absence of hydrocortisone. Whereas most SGPV genes were induced from day 3 along with the interferon-regulated innate immune response in gills, the putative SGPV virulence genes of the B22R family were expressed already one day after SGPV exposure, indicating a potential role as early markers of SGPV infection. In gills of the hydrocortisone-injected fish infected with SGPV, MX expression was delayed until day 10, and then expression skyrocketed along with the viral peak, gill pathology and mortality occurring from day 14. A similar expression pattern was observed for Interferon gamma (IFNγ) and granzyme A (GzmA) in the gills, indicating a role of acute cytotoxic cell activity in SGPVD. Duplex in situ hybridization demonstrated effects of hydrocortisone on the number and localization of GzmA-containing cells, and colocalization with SGPV infected cells in the gill. SGPV was generally not detected in spleen, and gill infection did not induce any corresponding systemic immune activity in the absence of stress hormone injection. However, in fish injected with hydrocortisone, IFNγ and GzmA gene expression was induced in spleen in the days prior to acute mortality. These data indicate that suppressed mucosal immune response in the gills and the late triggered systemic immune response in the spleen following hormonal stress induction may be the key to the onset of clinical SGPVD.en_US
dc.identifier.citationAmundsen M, Tartor HM, Andersen, Sveinsson KO, Thoen E, Gjessing MCG, Dahle MK. Mucosal and systemic immune responses to salmon gill poxvirus infection in Atlantic salmon is modulated upon hydrocortisone injection . Frontiers in Immunology. 2021;12en_US
dc.identifier.cristinIDFRIDAID 1912406
dc.identifier.doi10.3389/fimmu.2021.689302
dc.identifier.issn1664-3224
dc.identifier.urihttps://hdl.handle.net/10037/21406
dc.language.isoengen_US
dc.publisherFrontiers Mediaen_US
dc.relation.journalFrontiers in Immunology
dc.relation.projectIDinfo:eu-repo/grantAgreement/RCN/HAVBRUK2/267491/Norway/Understanding Salmon gill poxvirus disease; an emerging threat for Atlantic salmon farming//en_US
dc.relation.projectIDinfo:eu-repo/grantAgreement/RCN/HAVBRUK2/303415/Norway/Immune responses and survival in Salmon Gill Pox virus disease/IMMUNOPOX/en_US
dc.rights.accessRightsopenAccessen_US
dc.rights.holderCopyright 2021 The Author(s)en_US
dc.subjectVDP::Mathematics and natural science: 400::Zoology and botany: 480en_US
dc.subjectVDP::Matematikk og Naturvitenskap: 400::Zoologiske og botaniske fag: 480en_US
dc.titleMucosal and systemic immune responses to salmon gill poxvirus infection in Atlantic salmon is modulated upon hydrocortisone injectionen_US
dc.type.versionpublishedVersionen_US
dc.typeJournal articleen_US
dc.typeTidsskriftartikkelen_US
dc.typePeer revieweden_US


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