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dc.contributor.authorBarbieri, Liliana
dc.contributor.authorColin-York, Huw
dc.contributor.authorKorobchevskaya, Kseniya
dc.contributor.authorLi, Di
dc.contributor.authorWolfson, Deanna
dc.contributor.authorKaredla, Narain
dc.contributor.authorSchneider, Falk
dc.contributor.authorAhluwalia, Balpreet Singh
dc.contributor.authorSeternes, Tore
dc.contributor.authorDalmo, Roy Ambli
dc.contributor.authorDustin, Michael L.
dc.contributor.authorLi, Dong
dc.contributor.authorFritzsche, Marco
dc.date.accessioned2021-06-21T07:47:45Z
dc.date.available2021-06-21T07:47:45Z
dc.date.issued2021-04-12
dc.description.abstractQuantifying small, rapidly evolving forces generated by cells is a major challenge for the understanding of biomechanics and mechanobiology in health and disease. Traction force microscopy remains one of the most broadly applied force probing technologies but typically restricts itself to slow events over seconds and micron-scale displacements. Here, we improve >2-fold spatially and >10-fold temporally the resolution of planar cellular force probing compared to its related conventional modalities by combining fast two-dimensional total internal reflection fluorescence super-resolution structured illumination microscopy and traction force microscopy. This live-cell 2D TIRF-SIM-TFM methodology offers a combination of spatio-temporal resolution enhancement relevant to forces on the nano- and sub-second scales, opening up new aspects of mechanobiology to analysis.en_US
dc.identifier.citationBarbieri, Colin-York, Korobchevskaya, Li, Wolfson, Karedla, Schneider, Ahluwalia, Seternes, Dalmo, Dustin, Li, Fritzsche. Two-dimensional TIRF-SIM–traction force microscopy (2D TIRF-SIM-TFM). Nature Communications. 2021en_US
dc.identifier.cristinIDFRIDAID 1907219
dc.identifier.doi10.1038/s41467-021-22377-9
dc.identifier.issn2041-1723
dc.identifier.urihttps://hdl.handle.net/10037/21488
dc.language.isoengen_US
dc.publisherSpringer Natureen_US
dc.relation.journalNature Communications
dc.relation.projectIDinfo:eu-repo/grantAgreement/RCN/NANO2021/301401/Norway/Nano- and microplastics: Do they impact fish health and welfare?//en_US
dc.rights.accessRightsopenAccessen_US
dc.rights.holderCopyright 2021 The Author(s)en_US
dc.subjectVDP::Mathematics and natural science: 400::Basic biosciences: 470::Cell biology: 471en_US
dc.subjectVDP::Matematikk og Naturvitenskap: 400::Basale biofag: 470::Cellebiologi: 471en_US
dc.titleTwo-dimensional TIRF-SIM–traction force microscopy (2D TIRF-SIM-TFM)en_US
dc.type.versionpublishedVersionen_US
dc.typeJournal articleen_US
dc.typeTidsskriftartikkelen_US
dc.typePeer revieweden_US


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