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Transcriptome profiling of staphylococcus aureus associated extracellular vesicles reveals presence of small RNA-cargo

Permanent link
https://hdl.handle.net/10037/21704
DOI
https://doi.org/10.3389/fmolb.2020.566207
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Date
2021-01-13
Type
Journal article
Tidsskriftartikkel
Peer reviewed

Author
Joshi, Bishnu; Singh, Bhupender; Nadeem, Aftab; Askarian, Fatemeh; Wai, Sun Nyunt; Johannessen, Mona; Hegstad, Kristin
Abstract
Bacterial extracellular vesicles (EVs) have a vital role in bacterial pathogenesis. However, to date, the small RNA-cargo of EVs released by the opportunistic pathogen Staphylococcus aureus has not been characterized. Here, we shed light on the association of small RNAs with EVs secreted by S. aureus MSSA476 cultured in iron-depleted bacteriologic media supplemented with a subinhibitory dosage of vancomycin to mimic infection condition. Confocal microscopy analysis on intact RNase-treated EVs indicated that RNA is associated with EV particles. Transcriptomic followed by bioinformatics analysis of EV-associated RNA revealed the presence of potential gene regulatory small RNAs and high levels of tRNAs. Among the EV-associated enriched small RNAs were SsrA, RsaC and RNAIII. Our finding invites new insights into the potential role of EV-associated RNA as a modulator of host-pathogen interaction. Introduction
Publisher
Frontiers Media
Citation
Joshi, Singh, Nadeem, Askarian, Wai, Johannessen, Hegstad. Transcriptome profiling of staphylococcus aureus associated extracellular vesicles reveals presence of small RNA-cargo. Frontiers in Molecular Biosciences. 2021;7:566207:1-16
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