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dc.contributor.authorJoshi, Bishnu
dc.contributor.authorSingh, Bhupender
dc.contributor.authorNadeem, Aftab
dc.contributor.authorAskarian, Fatemeh
dc.contributor.authorWai, Sun Nyunt
dc.contributor.authorJohannessen, Mona
dc.contributor.authorHegstad, Kristin
dc.date.accessioned2021-07-02T13:48:37Z
dc.date.available2021-07-02T13:48:37Z
dc.date.issued2021-01-13
dc.description.abstractBacterial extracellular vesicles (EVs) have a vital role in bacterial pathogenesis. However, to date, the small RNA-cargo of EVs released by the opportunistic pathogen Staphylococcus aureus has not been characterized. Here, we shed light on the association of small RNAs with EVs secreted by S. aureus MSSA476 cultured in iron-depleted bacteriologic media supplemented with a subinhibitory dosage of vancomycin to mimic infection condition. Confocal microscopy analysis on intact RNase-treated EVs indicated that RNA is associated with EV particles. Transcriptomic followed by bioinformatics analysis of EV-associated RNA revealed the presence of potential gene regulatory small RNAs and high levels of tRNAs. Among the EV-associated enriched small RNAs were SsrA, RsaC and RNAIII. Our finding invites new insights into the potential role of EV-associated RNA as a modulator of host-pathogen interaction. Introductionen_US
dc.identifier.citationJoshi, Singh, Nadeem, Askarian, Wai, Johannessen, Hegstad. Transcriptome profiling of staphylococcus aureus associated extracellular vesicles reveals presence of small RNA-cargo. Frontiers in Molecular Biosciences. 2021;7:566207:1-16en_US
dc.identifier.cristinIDFRIDAID 1889148
dc.identifier.doi10.3389/fmolb.2020.566207
dc.identifier.issn2296-889X
dc.identifier.urihttps://hdl.handle.net/10037/21704
dc.language.isoengen_US
dc.publisherFrontiers Mediaen_US
dc.relation.journalFrontiers in Molecular Biosciences
dc.rights.accessRightsopenAccessen_US
dc.rights.holderCopyright 2021 The Author(s)en_US
dc.subjectVDP::Medical disciplines: 700::Basic medical, dental and veterinary science disciplines: 710en_US
dc.subjectVDP::Medisinske Fag: 700::Basale medisinske, odontologiske og veterinærmedisinske fag: 710en_US
dc.titleTranscriptome profiling of staphylococcus aureus associated extracellular vesicles reveals presence of small RNA-cargoen_US
dc.type.versionpublishedVersionen_US
dc.typeJournal articleen_US
dc.typeTidsskriftartikkelen_US
dc.typePeer revieweden_US


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