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dc.contributor.authorValentini, Alice
dc.contributor.authorSoininen, Eeva Marjatta
dc.contributor.authorCoissac, Eric
dc.contributor.authorIms, Rolf Anker
dc.contributor.authorMiquel, Christian
dc.contributor.authorYoccoz, Nigel Gilles
dc.contributor.authorGielly, Ludovic
dc.contributor.authorBrochmann, Christian
dc.contributor.authorBrysting, Anne K.
dc.contributor.authorSønstebø, Jørn H.
dc.contributor.authorTaberlet, Pierre
dc.date.accessioned2009-10-19T12:48:41Z
dc.date.available2009-10-19T12:48:41Z
dc.date.issued2009-08-20
dc.description.abstractBackground: In order to understand the role of herbivores in trophic webs, it is essential to know what they feed on. Diet analysis is, however, a challenge in many small herbivores with a secretive life style. In this paper, we compare novel (high-throughput pyrosequencing) DNA barcoding technology for plant mixture with traditional microhistological method. We analysed stomach contents of two ecologically important subarctic vole species, <i>Microtus oeconomus</i> and <i>Myodes rufocanus</i>, with the two methods. DNA barcoding was conducted using the P6-loop of the chloroplast <i>trn</i>L (UAA) intron. <br> Results: Although the identified plant taxa in the diets matched relatively well between the two methods, DNA barcoding gave by far taxonomically more detailed results. Quantitative comparison of results was difficult, mainly due to low taxonomic resolution of the microhistological method, which also in part explained discrepancies between the methods. Other discrepancies were likely due to biases mostly in the microhistological analysis. <br> Conclusion: We conclude that DNA barcoding opens up for new possibilities in the study of plant-herbivore interactions, giving a detailed and relatively unbiased picture of food utilization of herbivores.en
dc.format.extent250844 bytes
dc.format.mimetypeapplication/pdf
dc.identifier.citationFrontiers in Zoology 2009, 6:16en
dc.identifier.urihttps://hdl.handle.net/10037/2195
dc.identifier.urnURN:NBN:no-uit_munin_1947
dc.language.isoengen
dc.publisherBioMed Centralen
dc.rights.accessRightsopenAccess
dc.subjectVDP::Mathematics and natural science: 400en
dc.titleAnalysing diet of small herbivores : the efficiency of DNA barcoding coupled with high-throughput pyrosequencing for deciphering the composition of complex plant mixturesen
dc.typeJournal articleen
dc.typeTidsskriftartikkelen
dc.typePeer revieweden


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