Show simple item record

dc.contributor.authorAL-Rubaye, Mushtaq Talib Shawi
dc.contributor.authorJanice, Jessin
dc.contributor.authorBjørnholt, Jørgen Vildershøj
dc.contributor.authorJakovljev, Aleksandra
dc.contributor.authorHultström, Maria Elisabeth
dc.contributor.authorSundsfjord, Arnfinn
dc.contributor.authorHegstad, Kristin
dc.date.accessioned2021-08-11T08:26:12Z
dc.date.available2021-08-11T08:26:12Z
dc.date.issued2021-07-23
dc.description.abstractBackground - Vancomycin-resistant enterococci (VRE) represent several types of transferable vancomycin resistance gene clusters. The vanD type, associated with moderate to high level vancomycin resistance, has only sporadically been described in clinical isolates. The aim of this study was to perform a genetic characterization of the first VanD-type VRE strains detected in Norway.<p> <p>Methods - The VanD-type VRE-strains (n = 6) from two patient cases were examined by antimicrobial susceptibility testing and whole genome sequencing (WGS) to uncover Van-phenotype, strain phylogeny, the vanD gene clusters, and their genetic surroundings. The putative transferability of vanD was examined by circularization PCR and filter mating.<p> <p>Results - The VanD-type Enterococcus faecium (n = 4) and Enterococcus casseliflavus (n = 2) strains recovered from two cases (A and B), expressed moderate to high level vancomycin resistance (MIC 64—>256 mg/L) and various levels of teicoplanin susceptibility (MIC 2—>256 mg/L). WGS analyses revealed phylogenetically different E. faecium strains (A1, A2, and A3 of case A and B1 from case B) as well as vanD gene clusters located on different novel genomic islands (GIs). The E. casseliflavus strains (B2 and B3 of case B) were not clonally related, but harbored nearly identical novel GIs. The vanD cluster of case B strains represents a novel vanD-subtype. All the vanD-GIs were integrated at the same chromosomal site and contained genes consistent with a Clostridiales origin. Circular forms of the vanD-GIs were detected in all strains except B1. Transfer of vanD to an E. faecium recipient was unsuccessful.<p> <p>Conclusions - We describe the first VanD-type E. casseliflavus strains, a novel vanD-subtype, and three novel vanD-GIs with a genetic content consistent with a Clostridiales order origin. Despite temporal occurrence, case A and B E. faecium strains were phylogenetically diverse and harbored different vanD subtypes and vanD-GIs.en_US
dc.identifier.citationAl-Rubaye MTS, Janice JJ, Bjørnholt, Jakovljev, Hultström Me, Sundsfjord, Hegstad. Novel genomic islands and a new vanDsubtype in the first sporadic VanD-type vancomycin resistant enterococci in Norway. PLOS ONE. 2021;16(7):1-15en_US
dc.identifier.cristinIDFRIDAID 1924187
dc.identifier.doi10.1371/journal.pone.0255187
dc.identifier.issn1932-6203
dc.identifier.urihttps://hdl.handle.net/10037/21992
dc.language.isoengen_US
dc.publisherPublic Library of Scienceen_US
dc.relation.ispartofAL-Rubaye, M.T.S. (2023). Exploring the genomes of the Norwegian vancomycin resistant enterococci. (Doctoral thesis). <a href=https://hdl.handle.net/10037/28673>https://hdl.handle.net/10037/28673</a>.
dc.relation.journalPLOS ONE
dc.rights.accessRightsopenAccessen_US
dc.rights.holderCopyright 2021 The Author(s)en_US
dc.subjectVDP::Mathematics and natural science: 400::Zoology and botany: 480en_US
dc.subjectVDP::Matematikk og Naturvitenskap: 400::Zoologiske og botaniske fag: 480en_US
dc.titleNovel genomic islands and a new vanDsubtype in the first sporadic VanD-type vancomycin resistant enterococci in Norwayen_US
dc.type.versionpublishedVersionen_US
dc.typeJournal articleen_US
dc.typeTidsskriftartikkelen_US
dc.typePeer revieweden_US


File(s) in this item

Thumbnail

This item appears in the following collection(s)

Show simple item record