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dc.contributor.authorJanice, Jessin
dc.contributor.authorAgyepong, Nicholas
dc.contributor.authorOwusu-Ofori, Alex
dc.contributor.authorGovinden, Usha
dc.contributor.authorEssack, Sabiha
dc.contributor.authorSamuelsen, Ørjan
dc.contributor.authorSundsfjord, Arnfinn
dc.contributor.authorPedersen, Torunn
dc.date.accessioned2021-10-26T09:18:56Z
dc.date.available2021-10-26T09:18:56Z
dc.date.issued2021-06-17
dc.description.abstractTwo novel bla<sub>DIM-1</sub>- or bla<SUB>IMP-1</SUB>-containing genomic islands (GIs) were discovered by whole-genome sequence analyses in four extensively drug-resistant (XDR) <i>Pseudomonas aeruginosa</i> isolates from inpatients at a tertiary hospital in Ghana. The strains were of sequence type 234 (ST234) and formed a phylogenetic clade together with ST111, which is recognized as a global high-risk clone. Their carbapenem resistance was encoded by two Tn<i>402</i>-type integrons, In1592 (<i>bla</i><sub>DIM-1</sub>) and In1595 (<i>bla</i><sub>IMP-1</sub>), both carrying complete <i>tni</i> mobilization modules. In1595 was bound by conserved 25-bp inverted repeats (IRs) flanked by 5-bp direct repeats (DRs) associated with target site duplication. The integrons were embedded in two GIs that contained cognate integrases and were distinguished by a lower GC content than the chromosomal average. PAGI-97A (52.659 bp; In1592), which encoded a P4-type site-specific integrase of the tyrosine recombinase family in its 3′ border, was integrated into tRNA-Pro(ggg) and bracketed by a 49-bp perfect DR created by 3′-end target duplication. GIs with the same structural features, but diverse genetic content, were identified in 41/226 completed <i>P. aeruginosa</i> genomes. PAGI-97B (22,636 bp; In1595), which encoded an XerC/D superfamily integrase in its 5′ border, was inserted into the small RNA (sRNA) PrrF1/PrrF2 locus. Specific insertions into this highly conserved locus involved in iron-dependent regulation, all leaving PrrF1 intact, were identified in an additional six phylogenetically unrelated <i>P. aeruginosa</i> genomes. Our molecular analyses unveiled a hospital-associated clonal dissemination of carbapenem-resistant ST234 <i>P. aeruginosa</i> in which the XDR phenotype resulted from novel insertions of two GIs into specific chromosomal sites.en_US
dc.identifier.citationJanice JJ, Agyepong, Owusu-Ofori, Govinden, Essack, Samuelsen, Sundsfjord, Pedersen. Carbapenem resistance determinants acquired through novel chromosomal integrations in extensively drug-resistant pseudomonas aeruginosa. Antimicrobial Agents and Chemotherapy. 2021;65(7):1-15en_US
dc.identifier.cristinIDFRIDAID 1921025
dc.identifier.doi10.1128/AAC.00289-21
dc.identifier.issn0066-4804
dc.identifier.issn1098-6596
dc.identifier.urihttps://hdl.handle.net/10037/22830
dc.language.isoengen_US
dc.publisherAmerican Society for Microbiologyen_US
dc.relation.journalAntimicrobial Agents and Chemotherapy
dc.rights.accessRightsopenAccessen_US
dc.rights.holderCopyright 2021 The Author(s)en_US
dc.subjectVDP::Medical disciplines: 700::Basic medical, dental and veterinary science disciplines: 710en_US
dc.subjectVDP::Medisinske Fag: 700::Basale medisinske, odontologiske og veterinærmedisinske fag: 710en_US
dc.titleCarbapenem resistance determinants acquired through novel chromosomal integrations in extensively drug-resistant pseudomonas aeruginosaen_US
dc.type.versionpublishedVersionen_US
dc.typeJournal articleen_US
dc.typeTidsskriftartikkelen_US
dc.typePeer revieweden_US


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