Show simple item record

dc.contributor.authorMaharajan, Amudha Deepalakshmi
dc.contributor.authorHansen, Hilde
dc.contributor.authorKhider, Miriam
dc.contributor.authorWillassen, Nils P
dc.date.accessioned2021-11-25T09:32:49Z
dc.date.available2021-11-25T09:32:49Z
dc.date.issued2021-08-24
dc.description.abstractBackground Quorum Sensing (QS) is a cell-to-cell communication system that bacteria utilize to adapt to the external environment by synthesizing and responding to signalling molecules called autoinducers. The psychrotrophic bacterium Aliivibrio wodanis 06/09/139, originally isolated from a winter ulcer of a reared Atlantic salmon, produces the autoinducer N-3-hydroxy-decanoyl-homoserine-lactone (3OHC10-HSL) and encodes the QS systems AinS/R and LuxS/PQ, and the master regulator LitR. However, the role of QS in this bacterium has not been investigated yet. Results In the present work we show that 3OHC10-HSL production is cell density and temperature-dependent in A. wodanis 06/09/139 with the highest production occurring at a low temperature (6 °C). Gene inactivation demonstrates that AinS is responsible for 3OHC10-HSL production and positively regulated by LitR. Inactivation of ainS and litR further show that QS is involved in the regulation of growth, motility, hemolysis, protease activity and siderophore production. Of these QS regulated activities, only the protease activity was found to be independent of LitR. Lastly, supernatants harvested from the wild type and the ΔainS and ΔlitR mutants at high cell densities show that inactivation of QS leads to a decreased cytopathogenic effect (CPE) in a cell culture assay, and strongest attenuation of the CPE was observed with supernatants harvested from the ΔlitR mutant. Conclusion A. wodanis 06/09/139 use QS to regulate a number of activities that may prove important for host colonization or interactions. The temperature of 6 °C that is in the temperature range at which winter ulcer occurs, plays a role in AHL production and development of CPE on a Chinook Salmon Embryo (CHSE) cell line.en_US
dc.identifier.citationMaharajan, Hansen, Khider, Willassen. Quorum sensing in Aliivibrio wodanis 06/09/139 and its role in controlling various phenotypic traits. PeerJ. 2021en_US
dc.identifier.cristinIDFRIDAID 1929547
dc.identifier.doi10.7717/peerj.11980
dc.identifier.issn2167-8359
dc.identifier.urihttps://hdl.handle.net/10037/23167
dc.language.isoengen_US
dc.publisherPeerJen_US
dc.relation.ispartofMaharajan, A.D. (2023). AHL-mediated quorum sensing regulation: Role in controlling cytotoxicity, T6SSs and CRISPR-Cas systems in <i>Aliivibrio wodanis</i>. (Doctoral thesis). <a href=https://hdl.handle.net/10037/28075>https://hdl.handle.net/10037/28075</a>
dc.relation.journalPeerJ
dc.rights.accessRightsopenAccessen_US
dc.rights.holderCopyright 2021 The Author(s)en_US
dc.subjectVDP::Medical disciplines: 700::Basic medical, dental and veterinary science disciplines: 710en_US
dc.subjectVDP::Medisinske Fag: 700::Basale medisinske, odontologiske og veterinærmedisinske fag: 710en_US
dc.titleQuorum sensing in Aliivibrio wodanis 06/09/139 and its role in controlling various phenotypic traitsen_US
dc.type.versionpublishedVersionen_US
dc.typeJournal articleen_US
dc.typeTidsskriftartikkelen_US
dc.typePeer revieweden_US


File(s) in this item

Thumbnail

This item appears in the following collection(s)

Show simple item record