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dc.contributor.authorEdholm, Eva-Stina
dc.contributor.authorFenton, Christopher Graham
dc.contributor.authorMondot, Stanislas
dc.contributor.authorPaulssen, Ruth H
dc.contributor.authorLefranc, Marie-Paule
dc.contributor.authorBoudinot, Pierre
dc.contributor.authorMagadan, Susana
dc.date.accessioned2021-12-21T12:09:34Z
dc.date.available2021-12-21T12:09:34Z
dc.date.issued2021-10-18
dc.description.abstractIn jawed vertebrates, two major T cell populations have been characterized. They are defined as α/β or γ/δ T cells, based on the expressed T cell receptor. Salmonids (family Salmonidae) include two key teleost species for aquaculture, rainbow trout (Oncorhynchus mykiss) and Atlantic salmon (Salmo salar) which constitute important models for fish immunology and important targets for vaccine development. The growing interest to decipher the dynamics of adaptive immune responses against pathogens or vaccines has resulted in recent efforts to sequence the immunoglobulin (IG) or antibodies and T cell receptor (TR) repertoire in these species. In this context, establishing a comprehensive and coherent locus annotation is the fundamental basis for the analysis of high-throughput repertoire sequencing data. We therefore decided to revisit the description and annotation of TRA/TRD locus in Atlantic salmon and two strains of rainbow trout (Swanson and Arlee) using the now available high-quality genome assemblies. Phylogenetic analysis of functional TRA/TRD V genes from these three genomes led to the definition of 25 subgroups shared by both species, some with particular feature. A total of 128 TRAJ genes were identified in Salmo, the majority with a close counterpart in Oncorhynchus. Analysis of expressed TRA repertoire indicates that most TRAV gene subgroups are expressed at mucosal and systemic level. The present work on TRA/TRD locus annotation along with the analysis of TRA repertoire sequencing data show the feasibility and advantages of a common salmonid TRA/TRD nomenclature that allows an accurate annotation and analysis of high-throughput sequencing results, across salmonid T cell subsets.en_US
dc.identifier.citationEdholm, Fenton, Mondot, Paulssen, Lefranc, Boudinot, Magadan. Profiling the T Cell Receptor Alpha/Delta Locus in Salmonids. Frontiers in Immunology. 2021;12:1-17en_US
dc.identifier.cristinIDFRIDAID 1940398
dc.identifier.doi10.3389/fimmu.2021.753960
dc.identifier.issn1664-3224
dc.identifier.urihttps://hdl.handle.net/10037/23460
dc.language.isoengen_US
dc.publisherFrontiers Mediaen_US
dc.relation.journalFrontiers in Immunology
dc.relation.projectIDinfo:eu-repo/grantAgreement/RCN/HAVBRUK2/295036/Norway/CRISPRized Immortality– Novel approaches to immortalize fish cell lines - 3Rs//en_US
dc.relation.projectIDinfo:eu-repo/grantAgreement/EC/FP7/311993/Norway/Targeted disease prophylaxis in European fish farming/TARGETFISH/en_US
dc.rights.accessRightsopenAccessen_US
dc.rights.holderCopyright 2021 The Author(s)en_US
dc.subjectVDP::Mathematics and natural science: 400::Zoology and botany: 480en_US
dc.subjectVDP::Matematikk og Naturvitenskap: 400::Zoologiske og botaniske fag: 480en_US
dc.titleProfiling the T Cell Receptor Alpha/Delta Locus in Salmonidsen_US
dc.type.versionpublishedVersionen_US
dc.typeJournal articleen_US
dc.typeTidsskriftartikkelen_US
dc.typePeer revieweden_US


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