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dc.contributor.authorKhezri, Abdolrahman
dc.contributor.authorAvershina, Ekaterina
dc.contributor.authorAhmad, Rafi
dc.date.accessioned2022-01-25T13:33:53Z
dc.date.available2022-01-25T13:33:53Z
dc.date.issued2021-12-10
dc.description.abstractEmerging new sequencing technologies have provided researchers with a unique opportunity to study factors related to microbial pathogenicity, such as antimicrobial resistance (AMR) genes and virulence factors. However, the use of whole-genome sequence (WGS) data requires good knowledge of the bioinformatics involved, as well as the necessary techniques. In this study, a total of nine Escherichia coli and Klebsiella pneumoniae isolates from Norwegian clinical samples were sequenced using both MinION and Illumina platforms. Three out of nine samples were sequenced directly from blood culture, and one sample was sequenced from a mixed-blood culture. For genome assembly, several long-read, (Canu, Flye, Unicycler, and Miniasm), short-read (ABySS, Unicycler and SPAdes) and hybrid assemblers (Unicycler, hybridSPAdes, and MaSurCa) were tested. Assembled genomes from the best-performing assemblers (according to quality checks using QUAST and BUSCO) were subjected to downstream analyses. Flye and Unicycler assemblers performed best for the assembly of long and short reads, respectively. For hybrid assembly, Unicycler was the top-performing assembler and produced more circularized and complete genome assemblies. Hybrid assembled genomes performed substantially better in downstream analyses to predict putative plasmids, AMR genes and β-lactamase gene variants, compared to MinION and Illumina assemblies. Thus, hybrid assembly has the potential to reveal factors related to microbial pathogenicity in clinical and mixed samples.en_US
dc.identifier.citationKhezri A, Avershina E, Ahmad R. Hybrid Assembly Provides Improved Resolution of Plasmids, Antimicrobial Resistance Genes, and Virulence Factors in Escherichia coli and Klebsiella pneumoniae Clinical Isolates. Microorganisms. 2021;9(12):1-18en_US
dc.identifier.cristinIDFRIDAID 1967679
dc.identifier.doi10.3390/microorganisms9122560
dc.identifier.issn2076-2607
dc.identifier.urihttps://hdl.handle.net/10037/23800
dc.language.isoengen_US
dc.publisherMDPIen_US
dc.relation.journalMicroorganisms
dc.relation.projectIDinfo:eu-repo/grantAgreement/RCN/BEDREHELSE/ 273609/Norway/AMR-Diag: A Novel Diagnostic Tool for Sequence Based Prediction of Antimicrobial Resistance//en_US
dc.rights.accessRightsopenAccessen_US
dc.rights.holderCopyright 2021 The Author(s)en_US
dc.titleHybrid Assembly Provides Improved Resolution of Plasmids, Antimicrobial Resistance Genes, and Virulence Factors in Escherichia coli and Klebsiella pneumoniae Clinical Isolatesen_US
dc.type.versionpublishedVersionen_US
dc.typeJournal articleen_US
dc.typeTidsskriftartikkelen_US
dc.typePeer revieweden_US


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