Show simple item record

dc.contributor.authorNilsen, Bo Wold
dc.contributor.authorSimon-Santamaria, Jaione
dc.contributor.authorØrtengren, Ulf Thore
dc.contributor.authorJensen, Einar
dc.contributor.authorBruun, Jack-Ansgar
dc.contributor.authorMichelsen, Vibeke Barman
dc.contributor.authorSørensen, Karen Kristine
dc.date.accessioned2022-05-03T08:10:20Z
dc.date.available2022-05-03T08:10:20Z
dc.date.issued2018-07-27
dc.description.abstractTriethylene glycol dimethacrylate (TEGDMA) is commonly used in polymer resinbased dental materials. This study investigated the molecular mechanisms of TEGDMA toxicity by identifying its time- and dose-dependent effects on the proteome of human THP-1 monocytes. The effects of different concentrations (0.07– 5 mM) and exposure times (0–72 h) of TEGDMA on cell viability, proliferation, and morphology were determined using a real-time viability assay, automated cell counting, and electron microscopy, and laid the fundament for choice of exposure scenarios in the proteomic experiments. Solvents were not used, as TEGDMA is soluble in cell culture medium (determined by photon correlation spectroscopy). Cells were metabolically labeled [using the stable isotope labeled amino acids in cell culture (SILAC) strategy], and exposed to 0, 0.3 or 2.5 mM TEGDMA for 6 or 16 h before liquid chromatography-tandem mass spectrometry (LC-MS/MS) analyses. Regulated proteins were analyzed in the STRING database. Cells exposed to 0.3 mM TEGDMA showed increased viability and time-dependent upregulation of proteins associated with stress/oxidative stress, autophagy, and cytoprotective functions. Cells exposed to 2.5 mM TEGDMA showed diminished viability and a protein expression profile associated with oxidative stress, DNA damage, mitochondrial dysfunction, and cell cycle inhibition. Altered expression of immune genes was observed in both groups. The study provides novel knowledge about TEGDMA toxicity at the proteomic level. Of note, even low doses of TEGDMA induced a substantial cellular response.en_US
dc.identifier.citationNilsen B, Simon-Santamaria J, Ørtengren UT, Jensen e, Bruun JA, Michelsen VB, Sørensen KK. Dose- and time-dependent effects of triethylene glycol dimethacrylate on the proteome of human THP-1 monocytes. European Journal of Oral Sciences. 2018;126(5):345-358en_US
dc.identifier.cristinIDFRIDAID 1615320
dc.identifier.doi10.1111/eos.12559
dc.identifier.issn0909-8836
dc.identifier.issn1600-0722
dc.identifier.urihttps://hdl.handle.net/10037/24979
dc.language.isoengen_US
dc.publisherWileyen_US
dc.relation.journalEuropean Journal of Oral Sciences
dc.rights.accessRightsopenAccessen_US
dc.rights.holderCopyright 2018 The Author(s)en_US
dc.titleDose- and time-dependent effects of triethylene glycol dimethacrylate on the proteome of human THP-1 monocytesen_US
dc.type.versionpublishedVersionen_US
dc.typeJournal articleen_US
dc.typeTidsskriftartikkelen_US
dc.typePeer revieweden_US


File(s) in this item

Thumbnail

This item appears in the following collection(s)

Show simple item record