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dc.contributor.authorTietze, Lisa
dc.contributor.authorHoff, Maria Wilhelmsen
dc.contributor.authorLale, Rahmi
dc.date.accessioned2022-05-12T10:39:32Z
dc.date.available2022-05-12T10:39:32Z
dc.date.issued2022-01-27
dc.description.abstractVibrio natriegens has recently gained attention as a novel fast-growing bacterium in synthetic biology applications. Currently, a limited set of genetic elements optimised for Escherichia coli are used in V. natriegens due to the lack of DNA parts characterised in this novel host. In this study, we report the identification and cross-characterisation of artificial promoters and 5′ untranslated regions (artificial regulatory sequence, ARES) that lead to production of fluorescent proteins with a wide-range of expression levels. We identify and cross-characterise 52 constructs in V. natriegens and E. coli. Furthermore, we report the DNA sequence and motif analysis of the ARESs using various algorithms. With this study, we expand the pool of characterised genetic DNA parts that can be used for different biotechnological applications using V. natriegens as a host microorganism.en_US
dc.identifier.citationTietze L, Hoff MW, Lale R. Identification and Cross-Characterisation of Artificial Promoters and 5′ Untranslated Regions in Vibrio natriegens. Frontiers in Bioengineering and Biotechnology. 2022en_US
dc.identifier.cristinIDFRIDAID 1997687
dc.identifier.doi10.3389/fbioe.2022.826142
dc.identifier.issn2296-4185
dc.identifier.urihttps://hdl.handle.net/10037/25101
dc.language.isoengen_US
dc.publisherFrontiers Mediaen_US
dc.relation.journalFrontiers in Bioengineering and Biotechnology
dc.rights.accessRightsopenAccessen_US
dc.rights.holderCopyright 2022 The Author(s)en_US
dc.titleIdentification and Cross-Characterisation of Artificial Promoters and 5′ Untranslated Regions in Vibrio natriegensen_US
dc.type.versionpublishedVersionen_US
dc.typeJournal articleen_US
dc.typeTidsskriftartikkelen_US
dc.typePeer revieweden_US


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