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dc.contributor.authorQuach, Huy Quang
dc.contributor.authorJohnson, Christina
dc.contributor.authorEkholt, Karin
dc.contributor.authorIslam, Rakibul
dc.contributor.authorMollnes, Tom Eirik
dc.contributor.authorNilsson, Per
dc.date.accessioned2022-08-31T07:23:54Z
dc.date.available2022-08-31T07:23:54Z
dc.date.issued2022-04-04
dc.description.abstractObjective: In a recent study, we found an elevated level of interleukin 8 (IL-8) in response to bacterial incubation in thrombin-sufficient human whole blood anticoagulated by the fibrin polymerization blocking peptide GPRP. Whether thrombin directly activated leukocytes or mediated the release via thrombin-dependent activation of platelets remains unresolved. Herein, we addressed the role of thrombin and platelets in IL-8 release.<p> <p>Methods: We separated platelets from whole blood using a combination of 0.7% (w/v) citrate and GPRP for attenuating the hemostatic response during the separation of platelets. Cytokine responses were compared in whole blood and platelet-depleted blood upon Escherichia coli incubation. Cytokine responses were also profiled with and without reconstitution of either platelets or the supernatant from activated platelets. <p>Results: Platelets were not activated during the separation process but responded to stimuli upon re-calcification. Plasma levels of IL-1b, IL-1Ra, IL-6, IL-8, IP-10, MIP-1a, and MIP-1b were significantly reduced in platelet-depleted blood compared to whole blood, but recovered in the presence of platelets, or with the supernatant of activated platelets. The leukocyte fraction and platelets were each found to contribute to the elevation of IL-8 at around 5 ng/ml; however, if combined, the release of IL-8 increased to 26 ng/ml. This process was dependent on thrombin since the levels of IL-8 remained at 5 ng/ml in whole blood if thrombin was blocked. Intracellular staining revealed that monocytes were the main source for IL-8 expression. <p>Conclusion: Our findings suggest that the release of IL-8 is mediated by the leukocytes, mainly monocytes, but potentiated via thrombin-dependent activation of platelets.en_US
dc.identifier.citationQuach, Johnson, Ekholt, Islam, Mollnes, Nilsson. Platelet-depletion of whole blood reveals that platelets potentiate the release of IL-8 from leukocytes Into plasma in a thrombin-dependent manner. Frontiers in Immunology. 2022;13:865386:1-10en_US
dc.identifier.cristinIDFRIDAID 2028888
dc.identifier.doi10.3389/fimmu.2022.865386
dc.identifier.issn1664-3224
dc.identifier.urihttps://hdl.handle.net/10037/26482
dc.language.isoengen_US
dc.publisherFrontiers Mediaen_US
dc.relation.journalFrontiers in Immunology
dc.rights.accessRightsopenAccessen_US
dc.rights.holderCopyright 2022 The Author(s)en_US
dc.titlePlatelet-depletion of whole blood reveals that platelets potentiate the release of IL-8 from leukocytes Into plasma in a thrombin-dependent manneren_US
dc.type.versionpublishedVersionen_US
dc.typeJournal articleen_US
dc.typeTidsskriftartikkelen_US
dc.typePeer revieweden_US


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