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dc.contributor.authorStröhl, Florian
dc.contributor.authorHansen, Daniel Henry
dc.contributor.authorNàger, Mireia
dc.contributor.authorBirgisdottir, Åsa birna
dc.date.accessioned2022-09-15T12:36:51Z
dc.date.available2022-09-15T12:36:51Z
dc.date.issued2022
dc.description.abstractMultifocus microscopy enables recording of entire volumes in a single camera 11 exposure. In dense samples, multifocus microscopy is severely hampered by background haze. 12 Here, we introduce a scalable multifocus method that incorporates optical sectioning and offers 13 improved axial resolution capabilities. In our method, a dithered oblique light-sheet scans the 14 sample volume during a single exposure, while fluorescence from each illuminated plane in the 15 sample is mapped onto a line on the camera with a multifocus optical element. A synchronized 16 rolling shutter readout realizes optical sectioning. We describe the technique theoretically and 17 verify its optical sectioning and resolution improvement capabilities. We demonstrate a 18 prototype system with a multifocus beam splitter cascade and record monolayers of endothelial 19 cells at 35 volumes per second. We furthermore image uncleared engineered human heart tissue 20 and visualize the distribution of mitochondria at high axial resolution. Our method manages to 21 capture sub-diffraction sized mitochondria-derived vesicles up to 30 µm deep into the tissue.en_US
dc.identifier.citationStröhl F, Hansen DH, Nàger M, Birgisdottir Åb. Multifocus microscopy with optical sectioning and high axial resolution. Optica. 2022en_US
dc.identifier.cristinIDFRIDAID 2051602
dc.identifier.doihttps://doi.org/10.1364/OPTICA.468583
dc.identifier.issn2334-2536
dc.identifier.urihttps://hdl.handle.net/10037/26803
dc.language.isoengen_US
dc.publisherOpticaen_US
dc.relation.journalOptica
dc.relation.projectIDinfo:eu-repo/grantAgreement/EC/H2020/964800/Norway/OrganVision: Technology for real-time visualizing and modelling of fundamental process in living organoids towards new insights into organ-specific health, disease, and recovery/OrganVision/en_US
dc.rights.accessRightsopenAccessen_US
dc.rights.holderCopyright 2022 The Author(s)en_US
dc.titleMultifocus microscopy with optical sectioning and high axial resolutionen_US
dc.type.versionacceptedVersionen_US
dc.typeJournal articleen_US
dc.typeTidsskriftartikkelen_US
dc.typePeer revieweden_US


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