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dc.contributor.authorRosenlund, Ingrid A
dc.contributor.authorCalin, George A.
dc.contributor.authorDragomir, Mihnea Paul
dc.contributor.authorKnutsen, Erik
dc.date.accessioned2022-10-26T10:05:25Z
dc.date.available2022-10-26T10:05:25Z
dc.date.issued2021-06-24
dc.description.abstractKnock-out (KO) of long non-coding RNAs (lncRNAs) enables functional characterization of this still poorly described group of transcripts. One of the most efficient and simplest methods to achieve complete KO of a lncRNA is by employing CRISPR/Cas gene editing. As most lncRNAs are not well annotated, their individual functional regions are not defined, and the majority of the transcripts are not affected by single nucleotide mutations. Therefore, CRISPR/Cas KO is more challenging for lncRNAs as compared to KO of protein coding genes. Strategies for lncRNAs KO include complete removal of the entire gene, removal of the promoter and transcriptional start site, abolishing exon-exon junctions, or removing the transcriptional termination site. Here, we describe the methodology to perform CRISPR/Cas9 KO of lncRNAs in vitro using electroporation as the method of transfection of pre-synthesized single guide RNAs (sgRNAs) and Cas9 enzyme.en_US
dc.identifier.citationRosenlund IA, Calin GA, Dragomir MP, Knutsen E: CRISPR/Cas9 to Silence Long Non-Coding RNAs. In: Navarro. Long Non-Coding RNAs in Cancer, 2021. Springeren_US
dc.identifier.cristinIDFRIDAID 1975397
dc.identifier.doi10.1007/978-1-0716-1581-2
dc.identifier.isbn978-1-0716-1580-5
dc.identifier.urihttps://hdl.handle.net/10037/27137
dc.language.isoengen_US
dc.publisherSpringeren_US
dc.rights.accessRightsopenAccessen_US
dc.rights.holderCopyright 2021 The Author(s)en_US
dc.titleCRISPR/Cas9 to Silence Long Non-Coding RNAsen_US
dc.type.versionacceptedVersionen_US
dc.typeChapteren_US
dc.typeBokkapittelen_US


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