Nuclease-Treated Stabilized Fermentation Product of Cetobacterium somerae Improves Growth, Non-specific Immunity, and Liver Health of Zebrafish (Danio rerio)
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https://hdl.handle.net/10037/27250Date
2022-07-06Type
Journal articleTidsskriftartikkel
Peer reviewed
Author
Xie, Mingxu; Hao, Qiang; Xia, Rui; Olsen, Rolf Erik; Ringø, Einar; Yang, Yalin; Zhang, Zhen; Ran, Chao; Zhou, ZhigangAbstract
High-fat diets (HFD) are harmful to fish health. Probiotics are commonly utilized to
improve fish nutrition metabolism, immune response, and health. Nucleic acids of the
probiotic bacterium can be hydrolyzed by nuclease to generate nucleotides. The present
study aimed to evaluate the effects of stabilized fermentation product of nucleasetreated Cetobacterium somerae XMX-1 [XMX-1 (N)] on growth, non-specific immunity,
and liver health of zebrafish (Danio rerio). Compared to the HFD group, 100 g/kg XMX1 (N) significantly increased weight gain and decreased feed conversion ratio (FCR).
However, 5 or 10 g/kg XMX-1 (N) had no influence on zebrafish growth. In addition,
supplementation of 100 g/kg XMX-1 (N) significantly increased lysozyme activity and
total antioxidant capacity in skin mucus, and the expression of inflammation related
genes interleukin 1 beta (IL-1β), interleukin 10 (IL-10), and interleukin 6 (IL-6) in the gut as
well as fatty acid oxidation related genes uncoupling protein 2 (UCP2) and proliferatoractivated receptor γ coactivator 1α (PGC1α) in the liver, while decreased the content
of hepatic triacylglycerol (TAG) in zebrafish. The gene sequencing, 16S rRNA, showed
that 100 g/kg XMX-1 (N) enhanced the relative abundance of Firmicutes while lowered
Proteobacteria and Actinobacteria. 10 g/kg XMX-1 (N) significantly increased lysozyme
activity and complement component 4 (C4) in skin mucus, and intestinal expression of
inflammation-related genes. In the 5 g/kg XMX-1 (N) group, however, only an increase
in C4 level in skin mucus was observed. Together, these results reveal that dietary
supplementation with nuclease-treated C. somerae XMX-1 (N) has a dose-dependent
beneficial effect on fish health.
Publisher
Frontiers MediaCitation
Xie, Hao, Xia, Olsen, Ringø, Yang, Zhang, Ran, Zhou. Nuclease-Treated Stabilized Fermentation Product of Cetobacterium somerae Improves Growth, Non-specific Immunity, and Liver Health of Zebrafish (Danio rerio). Frontiers in Nutrition. 2022;9Metadata
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