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dc.contributor.advisorStuge, Tor Brynjar
dc.contributor.advisorMørtberg, Trude Victoria
dc.contributor.advisorAhlen, Maria Therese
dc.contributor.authorSætre, Thomas
dc.date.accessioned2023-02-01T06:33:03Z
dc.date.available2023-02-01T06:33:03Z
dc.date.issued2022-02-01en
dc.description.abstractFetal- and neonatal alloimmune thrombocytopenia is a condition that can cause hemorrhage and potential brain damage or death in neonates. It is most often a result of maternal alloantibodies specific for fetal Human Platelet antigen 1a (HPA-1a) that destroy fetal platelets. The recognition of the HPA-1a peptide-MHC complex by T-cells involve a peculiar form of T-cell receptor peptide-MHC interaction in which the Leu33 residue is essential for T-cell stimulation, not by being directly presented to the TCR, but by acting as an anchor residue and increasing peptide binding and stability of the peptide-MHC complex, compared to the Pro33 allovariant. Studies of immunized women have been found to involve a diverse set of T-cells with varying responsiveness. To build a solid platform in which to study the peptide-MHC interactions involved in this condition, the goal of this project was to attempt to produce soluble, recombinant T-cell receptors from an HPA-1a-specific T cell line (D7T4) characterized from an alloimmunized woman. Through cloning and recombination of D7T4-TCRα and D7T4-TCRβ plasmids and vectors used in multigene baculoviral vectors, and their fusion, baculovirus vectors for transfection and expression of D7T4-TCRαβ in insect cells were produced. D7T4-TCRαβ was found to be expressed in its expected dimeric form in insect cells, but its functionality as a T-cell receptor was not clearly demonstrated.en_US
dc.identifier.urihttps://hdl.handle.net/10037/28452
dc.language.isoengen_US
dc.publisherUiT Norges arktiske universitetno
dc.publisherUiT The Arctic University of Norwayen
dc.rights.holderCopyright 2022 The Author(s)
dc.rights.urihttps://creativecommons.org/licenses/by-nc-sa/4.0en_US
dc.rightsAttribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)en_US
dc.subject.courseIDMED-3910
dc.subjectVDP::Medisinske Fag: 700::Basale medisinske, odontologiske og veterinærmedisinske fag: 710::Medisinsk immunologi: 716en_US
dc.subjectVDP::Medical disciplines: 700::Basic medical, dental and veterinary science disciplines: 710::Medical immunology: 716en_US
dc.titleRecombinant T-cell receptors from HPA-1a specific T-cellsen_US
dc.typeMastergradsoppgaveno
dc.typeMaster thesisen


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Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)
Except where otherwise noted, this item's license is described as Attribution-NonCommercial-ShareAlike 4.0 International (CC BY-NC-SA 4.0)